A8678

Agar

suitable for plant cell culture

• Synonym(s): Agar-agar, Gum agar
• Linear Formula: (C₁₂H₁₈O₉)_n
• CAS Number: 9002-18-0
• UNSPSC Code: 50405901
• NACRES: NA.21
• EC Number: 232-658-1
• MDL number: MFCD00081288

Properties

• biological source: algae (Rhodophyceae)
• Quality Level: 200
• form: powder
• technique(s): cell culture | plant: suitable
• mp: 80-90 °C (1.5% in water)
• transition temp: transition temp 27-37 °C (1.5%)
• application(s): agriculture
• storage temp.: room temp
• SMILES string: O1[C@@H]([C@@H]([C@@H]([C@H](C1OC2[C@H]3OCC2OC([C@H]3O)C)O)OC)O)CO
• InChI: 1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1
• InChI key: GYYDPBCUIJTIBM-DYOGSRDZSA-N

Description

General description

Agar or Agar-agar is one of the major components found in the cell wall of red algae. It is a polysaccharide complex, comprising about 70% agarose and 30% agaropectin.

Application

Washed agar has been used:

• as a supplement (0.9% w/v) to Murashige and Skoog basal medium for plant growth[1]
• in the preparation of cocultivation medium for Agrobacterium tumefaciens mediated transformation in maize (Zea mays) hybrid Hi-II[2]
• as a component in the complete medium to regenerate protoplasts of Piriformospora indica[3]
• as a clean agar medium to culture Synechococcus WH7803 strain[4]

Biochem/physiol Actions

Agar is an ester of sulfuric acid in a linear galactan form, that makes it soluble in hot water and insoluble in cold. Washed agar contain less impurities and low level of nutrients.

Preparation Note

Washed

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• ppe: Eyeshields, Gloves, type N95 (US)