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A6237

Apyrase from potato

recombinant, expressed in Pichia pastoris, ATPase ≥1000 units/mg protein, lyophilized powder

Synonym(s):

Apyrase from potato, Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Recombinant:
expressed in Pichia pastoris
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recombinant

expressed in Pichia pastoris

Quality Segment

form

lyophilized powder

ATPase activity

≥1000 units/mg protein

shipped in

wet ice

storage temp.

−20°C

General description

Apyrase, also known as ATP-diphosphohydrolase, is expressed in wide variety of plant and animal tissues. The commercially accessible apyrase enzyme is isolated from potato tubers.

Biochem/physiol Actions

Apyrase hydrolyses the phosphoanhydride bonds of nucleoside tri- and di-phosphates in the presence of divalent cations. It has wide substrate specificity for nucleotides. This property of the enzyme makes it suitable for different biotechnical applications, including DNA sequencing and platelet-aggregation inhibition.

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This Item
A2230A6410A6132
recombinant

expressed in Pichia pastoris

recombinant

-

recombinant

-

recombinant

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

-

Quality Level

200

Quality Level

-

Quality Level

200

Quality Level

300

ATPase activity

≥1000 units/mg protein

ATPase activity

≥600 units/mg protein

ATPase activity

≥200 units/mg protein

ATPase activity

≥3.0 units/mg protein


pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Global Trade Item Number

SKUGTIN
A6237-500UN04061832754611
A6237-100UN04061832754604

Questions

  1. Hello. Is the concentration of 100 units in 1 mL of water? If not, how can I make the dilution to obtain 100 units? Thanks for you attention! Guilherme

    1 answer
    1. The solubility of this enzyme is tested on the finished product at 1 mL per vial in water. The 100-unit package size will be readily soluble in 1 mL, however, the number of actual units per vial should be reviewed in the lot-specific certificate of analysis. The vial content is typically greater than 100 units. The reconstitution volume would have to be adjusted accordingly in order to achieve a 100 unit per milliliter concentration.
      See the link below to review the sample Certificate of analysis:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A6237-100UN0000174833.pdf

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