This product is not assigned an expiration or retest date. Products that are robust in nature and extremely stable, in the original unopened container, are not assigned an expiration date or retest schedule. These products will have no dating reported on either the label or the Certificate of Analysis. Products that are not assigned an expiration or retest date are covered by a warranty period of 1 year from the date of product shipment.
Full Product Dating Information may be reviewed at the link below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/190/408/product-dating-information-ms.pdf
A5222
D-Amino Acid Oxidase from porcine kidney
powder, ≥1.5 units/mg solid
Synonym(s):
D-AAO, D-Amino acid: oxygen oxidoreductase (deaminating)
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-563-5
MDL number:
Specific activity:
≥1.5 units/mg solid
Pricing and availability is not currently available.
Application
Other Notes
One unit will oxidatively deaminate 1.0 μmole of D-alanine to pyruvate per minute at pH 8.3 at 25 °C, in the presence of a catalase.
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This Item | |||
|---|---|---|---|
| specific activity ≥1.5 units/mg solid | specific activity ≥0.05 unit/mg solid | specific activity ≥4.0 units/mg protein | specific activity ≥60 units/mg protein |
| form powder | form solid | form aqueous suspension | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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What is the shelf life of this product at -20 °C?
1 answer-
Helpful?
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If I store it at -4 degrees Celsius instead of -20 degrees Celsius, will the enzyme denature so much that it cannot be used? Also, if I expose it to room temperature for use in an experiment and then store it at -4 degrees Celsius, will it be usable again
1 answer-
This product should be stored at -20 °C as a powder. Information on storing the product in solution is not available, and it is recommended to prepare the product fresh before use.
Helpful?
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Hello, I am doing my research about D-amino acid oxidase from porcine kidney. For further calculations I need molar extinction coefficient of pkDAAO. Maybe You have bigger description or some kind of information about it? Thank You.
1 answer-
There are two extinction coefficient values available:
Assuming all Cys residues form cystines - 1.935 at 280 nm in water, 0.1% (1 g/L)
Assuming all Cys residues are reduced - 1.929 at 280 nm in water, 0.1% (1 g/L)Please see the link below to review this and additional information available at Expasy:
https://web.expasy.org/cgi-bin/protparam/protparam1?P00371@noft@Helpful?
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