The volume of this product is lot specific and dependent upon the protein content and enzymatic activity. Volume may be determined using the specific activity value and mg protein/mL value reported in the Certificate of Analysis. For example, if the end-user has 500KU pack size and the specific activity is reported as 700 units/mg, while the concentration is 20.1 mg/ml. The volume would equate to roughly 35.52 mL. Please see the link below to review a sample or lot specific Certificate of Analysis:
https://www.sigmaaldrich.com/product/mm/cx2335#product-documentation
A3403
α-Amylase from Bacillus licheniformis
Type XII-A, saline solution, ≥500 units/mg protein (biuret)
Synonym(s):
Termamyl® 2X, 1,4-α-D-Glucan-glucanohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
MDL number:
Specific activity:
≥500 units/mg protein (biuret)
Pricing and availability is not currently available.
type
Type XII-A
Quality Level
form
saline solution
specific activity
≥500 units/mg protein (biuret)
mol wt
57.6 kDa
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
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sustainability
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greener alternative category
storage temp.
2-8°C
General description
α-Amylase is a member of the endo-amylase family. Its molecular weight is 57.6 kDa. α-Amylase is composed of three functional domains namely A, B and C. Domain A is a barrel-shaped (β/α)8 structure and is the largest domain. Domain B is linked to domain A via disulfide bond and is present between domain A and C. Domain C is composed of β-sheets and is connected to domain A via a polypeptide chain.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.
Application
α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. Product A3403 is from Bacillus licheniformis and is type XII-A. α-Amylase, from Sigma, has been used in various plant studies, such as metabolism studies in Arabidopsis [1].
Biochem/physiol Actions
α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose.
Physical form
Aqueous solution containing sodium chloride and sucrose
Other Notes
One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.
Legal Information
A product of Novozyme Corp.
Termamyl is a registered trademark of Novozymes Corp.
Disclaimer
Reported to be heat stable at temperatures as high as ∼90 °C.
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This Item | |||
|---|---|---|---|
| specific activity ≥500 units/mg protein (biuret) | specific activity - | specific activity 20000—60000 U/mL | specific activity 500-1,500 units/mg protein |
| form saline solution | form liquid | form solution | form lyophilized powder |
| mol wt 57.6 kDa | mol wt - | mol wt - | mol wt 58-62 kDa |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 100 | Quality Level 300 | Quality Level 200 |
| greener alternative product characteristics Waste Prevention | greener alternative product characteristics - | greener alternative product characteristics Waste Prevention | greener alternative product characteristics Waste Prevention |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Related Content
Instructions
Zsofia Botyanszki et al.
Biotechnology and bioengineering, 112(10), 2016-2024 (2015-05-08)
Biocatalytic transformations generally rely on purified enzymes or whole cells to perform complex transformations that are used on industrial scale for chemical, drug, and biofuel synthesis, pesticide decontamination, and water purification. However, both of these systems have inherent disadvantages related
Xinyi Huang et al.
Tree physiology, 40(5), 652-666 (2020-02-23)
Globally, soil salinization is becoming increasingly prevalent, due to local hydrogeologic phenomena, climate change and anthropogenic activities. This has significantly curtailed current world food production and limits future production potential. In the prairie region of North America, sulfate salts, rather
Michaelle Chokki et al.
Foods (Basel, Switzerland), 9(4) (2020-04-09)
Momordica charantia Linn. (Cucurbitaceae), the wild variety of bitter melon and Morinda lucida Benth (Rubiaceae) were commonly used as a popular folk medicine in Benin. This research focused to measure the antioxidant and enzyme inhibitory effects of M. charantia and
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A3403-500KU | 04061838251565 |
| A3403-5MU | 04061835553310 |
| A3403-1MU | 04061838251558 |
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What is the volume for the A3403-500KU
1 answer-
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What is the optimal conditions (pH and temperature) for this enzyme to degrade starch? Will it deactivate after heating to 100C?
1 answer-
This product has an optimum pH range of 5–9 and will maintain 98% of activity after 60 minutes at pH 6.2 at 85°C. This material is tested pH 6.9 at boiling temperature for 15 minutes. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/297/885/a3403pis.pdfHelpful?
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Dear Sir or Madam Could you tell me exactly in what solution the a-amylase is provided? Unfortunately only a "saline sucrose solution" is stated. I would need the exact composition in order to prepare a negative control in my assay. kind regards
1 answer-
All that we can disclose is that this product is supplied as an aqueous solution containing sodium chloride and sucrose with >=10 mg/mL protein.
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