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Rhodamine 110 chloride

Name: Rhodamine 110 chloride
Brand: SIGMA

suitable for fluorescence, BioReagent, ≥99.0% (UV)

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About This Item

Empirical Formula (Hill Notation):

C₂₀H₁₄N₂O₃ · HCl

CAS Number:

13558-31-1

Molecular Weight:

366.80

UNSPSC Code:

12171500

NACRES:

NA.32

PubChem Substance ID:

57652964

EC Number:

236-944-7

Beilstein/REAXYS Number:

4631860

MDL number:

MFCD00042009

Pricing and availability is not currently available.

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Properties

product line

BioReagent

Quality Level

200

assay

≥99.0% (UV)

form

crystals

mp

>300 °C (lit.)

solubility

ethanol: soluble, methanol: soluble

fluorescence

λ_ex 498 nm; λ_em 520 nm in methanol

suitability

suitable for fluorescence

SMILES string

Cl.Nc1ccc2c(OC3=CC(=N)C=CC3=C2c4ccccc4C(O)=O)c1

InChI

1S/C20H14N2O3.ClH/c21-11-5-7-15-17(9-11)25-18-10-12(22)6-8-16(18)19(15)13-3-1-2-4-14(13)20(23)24;/h1-10,21H,22H2,(H,23,24);1H

InChI key

JNGRENQDBKMCCR-UHFFFAOYSA-N

Description

General description

Rhodamine 110 or R110 is a triphenylmethane dye and emits a strong green-fluorescent light. The high fluorescence is attributed to the presence of amino groups as electron donating groups. Rhodamine 110 has excitation and emission maximum at 502.4/521.6 nm, respectively.[1]

Application

Rhodamine 110 is used for the determination of protease activity with accurate kinetic parameters.[2] It is used to measure caspase activity in cells with induced apoptosis.[3]

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1 of 1

This Item	432202	83694	83702
Sigma-Aldrich 83695 Rhodamine 110 chloride	Sigma-Aldrich 432202 Rhodamine 110 chloride	Sigma-Aldrich 83694 Rhodamine 101 inner salt	Sigma-Aldrich 83702 Rhodamine 123
suitability suitable for fluorescence	suitability -	suitability suitable for fluorescence	suitability -
fluorescence λ_ex 498 nm; λ_em 520 nm in methanol	fluorescence -	fluorescence λ_ex 560 nm; λ_em 589 nm in methanol	fluorescence -
form crystals	form powder or crystals	form solid	form powder
solubility ethanol: soluble, methanol: soluble	solubility -	solubility methanol: soluble	solubility ethanol: 20 mg/mL, clear, red
assay ≥99.0% (UV)	assay -	assay -	assay ≥85% (HPLC)
mp >300 °C (lit.)	mp >300 °C (lit.)	mp -	mp -

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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A fluorescence quenching method for the determination of nitrite with Rhodamine 110.

Xian Zhang et al.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 59(8), 1667-1672 (2003-05-09)

A simple and sensitive fluorescence quenching method for the determination of trace nitrite has been developed. The method is based on the reaction of Rhodamine 110 with nitrite in acidic medium to form a new compound, which has much lower

Diffusion coefficients of several rhodamine derivatives as determined by pulsed field gradient-nuclear magnetic resonance and fluorescence correlation spectroscopy.

P-O Gendron et al.

Journal of fluorescence, 18(6), 1093-1101 (2008-04-24)

Rhodamine derivatives are popular, photostable fluorophores that are used in a number of fluorescent based techniques, including fluorescence correlation spectroscopy (FCS). Indeed, in FCS, both rhodamine 6G (R6G) and rhodamine 110 (R110) are used as calibration standards to determine the

Rhodamine-based fluorogenic probe for imaging biological thiol.

Aya Shibata et al.

Bioorganic & medicinal chemistry letters, 18(7), 2246-2249 (2008-03-25)

We have developed a new fluorescent probe for biological thiol. The probe was synthesized by the modification of the 2,4-dinitrobenzenesulfonyl group with rhodamine 110. The selective detection of thiol species such as cysteine or glutathione was achieved in biological conditions.

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Global Trade Item Number

SKU	GTIN
83695-250MG	04061833278345

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