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18909

Calcofluor White Stain

suitable for microbiology

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About This Item

UNSPSC Code:
12171500
NACRES:
MA.02
Pricing and availability is not currently available.
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product line

BioChemika

Quality Level

form

liquid

composition

Calcofluor White M2R, 1 g/L , Evans blue, 0.5 g/L

technique(s)

microbe id | staining: suitable

color

blue to very dark blue

λmax

423-443 nm

fluorescence

λex 423-443 nm in 0.1 M phosphate pH 7.0

application(s)

food and beverages
hematology
histology, microbiology

storage temp.

room temp

suitability

fungi, parasites, yeasts

SMILES string

C1=CC=C(C=C1)NC2=NC(=NC(=N2)N(CCO)CCO)NC3=CC(=C(C=C3)C=CC4=C(C=C(C=C4)NC5=NC(=NC(=N5)NC6=CC=CC=C6)N(CCO)CCO)S(=O)(=O)O)S(=O)(=O)O

InChI

InChI=1S/C40H44N12O10S2/c53-21-17-51(18-22-54)39-47-35(41-29-7-3-1-4-8-29)45-37(49-39)43-31-15-13-27(33(25-31)63(57,58)59)11-12-28-14-16-32(26-34(28)64(60,61)62)44-38-46-36(42-30-9-5-2-6-10-30)48-40(50-38)52(19-23-55)20-24-56/h1-16,25-26,53-56H,17-24H2,(H,57,58,59)(H,60,61,62)(H2,41,43,45,47,49)(H2,42,44,46,48,50)/b12-11+

InChI key

CNGYZEMWVAWWOB-VAWYXSNFSA-N

General description

Calcofluor White Stain (C.l. 40622) is a stilbene compound that has additional triazinyl and phenyl aromatic groups. It is also known as cellufluor.

Application

Calcofluor White Stain is a fluorescent stain that is used for the rapid detection of yeasts, fungi, and parasitic organisms. It is a non-specific fluorochrome that binds to cellulose and chitin in cell walls. It is suitable for use as a viability stain for plant cells. Additionally, calcofluor white is recommended as a counterstain for in situ hybridization staining of plant material and as a flow cytometry stain for assessing the viability of animal cells.

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This Item
439254525321820
suitability

fungi, yeasts, parasites

suitability

-

suitability

-

suitability

-

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

color

blue to very dark blue

color

-

color

-

color

-

fluorescence

λex 423-443 nm in 0.1 M phosphate pH 7.0

fluorescence

-

fluorescence

-

fluorescence

-

form

liquid

form

liquid

form

-

form

liquid

application(s)

food and beverages
hematology
histology, microbiology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology


pictograms

Health hazardExclamation mark

signalword

Danger

hcodes

Hazard Classifications

Carc. 1B - Eye Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges



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Certificates of Analysis (COA)

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Nicholas R Thomson et al.
Genome research, 18(10), 1624-1637 (2008-06-28)
We have determined the complete genome sequences of a host-promiscuous Salmonella enterica serovar Enteritidis PT4 isolate P125109 and a chicken-restricted Salmonella enterica serovar Gallinarum isolate 287/91. Genome comparisons between these and other Salmonella isolates indicate that S. Gallinarum 287/91 is
Weiwei Dang et al.
Nature, 459(7248), 802-807 (2009-06-12)
Cells undergoing developmental processes are characterized by persistent non-genetic alterations in chromatin, termed epigenetic changes, represented by distinct patterns of DNA methylation and histone post-translational modifications. Sirtuins, a group of conserved NAD(+)-dependent deacetylases or ADP-ribosyltransferases, promote longevity in diverse organisms;
Yanqiu Zhao et al.
The New phytologist, 225(4), 1531-1544 (2019-07-02)
Wood formation is the terminal differentiation of xylem mother cells derived from cambial initials, and negative regulators play important roles in xylem differentiation. The molecular mechanism of the negative regulator of xylem differentiation PagKNAT2/6b was investigated. PagKNAT2/6b is an ortholog



Global Trade Item Number

SKUGTIN
440035-5G04061831810998
18909-100ML-F04061837829628

Questions

1–2 of 2 Questions  
  1. Can you tell us about the stability of the product once the bottle is opened?

    1 answer
    1. This Calcofluor White solution is prepared in water and packaged in an amber glass bottle. Provided the solution is not contaminated or allowed to evaporate, the product is expected to remain stable at least until the assigned retest date published in the lot-specific Certificate of Analysis.
      https://www.sigmaaldrich.com/product/sial/18909#product-documentation

      Helpful?

  2. Is this an aqueous solution? what is the solvent? I haven't been able to find that information.

    1 answer
    1. Yes, this product is an aqueous solution containing 1 g/L Calcofluor White and 0.5 g/L Evans Blue. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/177/132/18909bul-ms.pdf

      Helpful?

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