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About This Item
UNSPSC Code:
41105500
NACRES:
NA.54
Assay:
≥85% (HPLC)
Form:
solution
Storage temp.:
−20°C
Pricing and availability is not currently available.
assay
≥85% (HPLC)
form
solution
mol wt
Mr 1090.7
packaging
pkg of 125 μL (11558706910 [1 mM]), pkg of 5 × 125 μL (11570013910 [5x1 mM]), pkg of 25 μL (11093088910 [1 mM])
manufacturer/tradename
Roche
λmax
222 and 292 nm, 22430 and 8780
storage temp.
−20°C
General description
Digoxigenin-11-dUTP, alkali-stable is provided in 1 mM tetralithium salt, solution.
Application
Digoxigenin-11-dUTP, alkali-stable has been used to label probes for
fluorescent in situ hHybridization (FISH)[1] and polymerase chain reaction (PCR)[2]
Note: When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will remain attached. It is therefore ideal for use in DIG-labeled DNA that needs to survive exposure to alkaline treatment. However, if you intend to make a DIG-labeled probe that can be removed by alkali denaturation (e.g ., during stripping and reprobing of blots), do not use this alkali-stable preparation. Instead, use the alkali-labile form of DIG-11-dUTP.
fluorescent in situ hHybridization (FISH)[1] and polymerase chain reaction (PCR)[2]
Note: When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will remain attached. It is therefore ideal for use in DIG-labeled DNA that needs to survive exposure to alkaline treatment. However, if you intend to make a DIG-labeled probe that can be removed by alkali denaturation (e.g ., during stripping and reprobing of blots), do not use this alkali-stable preparation. Instead, use the alkali-labile form of DIG-11-dUTP.
Biochem/physiol Actions
Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) replaces deoxythymidine triphosphate (dTTP) in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% DIG-11-dUTP and 65% dTTP. DIG-11-dUTP can also replace dTTP in labeling during polymerase chain reaction (PCR) (e.g., with Taq DNA Polymerase), 3′-tailing (with terminal transferase) and cDNA synthesis (e.g., with transcriptor or M-MuLV reverse transcriptase). It is ideal as a substrate for DNA polymerase, Taq DNA polymerase, terminal transferase and reverse transcriptase.
Features and Benefits
Digoxigenin is bound to deoxyuridine triphosphate via an alkali-resistant ether linkage.
Resistance to alkali: The preparation is stable in the presence of 0.1 - 0.5M NaOH at +15 to +25°C.
Resistance to alkali: The preparation is stable in the presence of 0.1 - 0.5M NaOH at +15 to +25°C.
Preparation Note
Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).
Storage conditions (working solution): A decomposition of approx. 5% may occur within 4 weeks at 15 to 25 °C.
Storage conditions (working solution): A decomposition of approx. 5% may occur within 4 weeks at 15 to 25 °C.
Analysis Note
Typical analysis: At least 85% DIG-11-dUTP (HPLC, area%).
Function tested by RPL.
Function tested by RPL.
Other Notes
For life science research only. Not for use in diagnostic procedures.
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This Item | |||
|---|---|---|---|
| manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename Roche |
| assay ≥85% (HPLC) | assay 85% (HPLC) | assay 85% (HPLC) | assay ≥85% (HPLC) |
| form solution | form solution | form solution | form solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| packaging pkg of 125 μL (11558706910 [1 mM]), pkg of 25 μL (11093088910 [1 mM]), pkg of 5 × 125 μL (11570013910 [5x1 mM]) | packaging pkg of 125 μL (11573179910 [1 mM]]]), pkg of 25 μL (11573152910 [1 mM]) | packaging pkg of 25 μL (25 nmol; 1mM) | packaging pkg of 25 μL (11209256910 [10 mM]), pkg of 57 μL (03359247910 [3.5 mM]) |
| mol wt Mr 1090.7 | mol wt - | mol wt (Molecular weight: Mr = 1074.7 (DIG-11-ddUTP-Li4)) | mol wt (Mr = 1106.7 (DIG-11-UTP-Li4)) |
Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
does not flash
flash_point_c
does not flash
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 11570013910 | 04061837893452 |
| 11093088910 | 04061837893407 |
| 11558706910 | 04061837893445 |