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TMS-012

Dulbecco's Phosphate Buffered Saline

1X, liquid, w/o Ca++ & Mg++, Endotoxin-Free

Synonym(s):

DPBS, PBS, Phosphate buffered saline

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160405
NACRES:
NA.75
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Product Name

Endotoxin-Free Dulbecco′s PBS (1X) (w/o Ca++ & Mg++), Cell Culture

Quality Level

300

form

liquid

manufacturer/tradename

Chemicon®

technique(s)

cell culture | mammalian: suitable

General description

Endotoxin-Free Dulbecco’s PBS (1X) (w/o Ca++ & Mg++) is sterile filtered through a 0.1 micron filter and is considered endotoxin-free (<0.005 EU/ml).

Application

Endotoxin-Free Dulbecco′s PBS (1X) (w/o Ca++ & Mg++) has been used as a wash buffer during:
  • the isolation of colonic lamina propria cells
  • teratoma formation by induced pluripotent stem cells (iPSCs)
  • production of elastic porous injectable (EPI) biomaterial from the bulk scaffolds

Analysis Note

Appearance: Clear Liquid/No Particulates
Sterility Testing: Negative for Growth
pH: 7.0-7.2
Osmolality: 260-305 mOsm/kg
Endotoxin: <0.005 EU/mL
Appearance: Clear Liquid/No Particulates
Sterility Testing: Negative for Growth
pH: 7.0-7.2
Osmolality: 260-305 mOsm/kg
Endotoxin: <0.005 EU/mL

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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This Item
D40316506-OPPPB006
Endotoxin-Free Dulbecco&#8217;s PBS (1X) (w/o Ca++ &amp; Mg++) Cell Culture

Sigma-Aldrich

TMS-012

Endotoxin-Free Dulbecco′s PBS (1X) (w/o Ca++ & Mg++)

Dulbecco’s Phosphate Buffered Saline Modified, with 36&nbsp;mg sodium pyruvate, 50&nbsp;mg streptomycin sulfate, 100&nbsp;mg kanamycin monosulfate, 1000&nbsp;mg glucose/L and CaCl2, liquid, 0.1 &#956;m filtered, suitable for cell culture

Sigma-Aldrich

D4031

Dulbecco′s Phosphate Buffered Saline

Phosphate Buffered Saline OmniPur&#174; Grade, 10X Liquid Concentrate, Sterile

Millipore

6506-OP

Phosphate Buffered Saline

Phosphate buffered saline pH 7.4, pHast Pack&#8482;, powder

Sigma-Aldrich

PPB006

Phosphate buffered saline

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

Quality Level

300

Quality Level

200

Quality Level

100

Quality Level

-

form

liquid

form

liquid

form

liquid

form

powder

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Global Trade Item Number

SKUGTIN
TMS-012-A04055977052251

Questions

  1. Is this made with Bacteriostatic water? I need to reconstitute an enzyme with endotoxin free PBS but the enzyme is inhibited by bacteriostatic water.

    1 answer

    1. The method of preparation is considered proprietary. However, the term 'bacteriostatic' typically implies the presence of benzyl alcohol. No benzyl alcohol is present in the water used to prepare this product.
      The composition is as follows:
      0.2 g/L KCL
      0.2 g/L KH2PO4
      8.0 g/L NaCl
      1.15 g/L Na2HPO4 (anhydrous)

      With the pH adjusted to pH 7.0 to 7.1 using HCl.

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