Skip to Content
Merck

Key Documents

View All Documentation

Skip To

71456

BugBuster® Master Mix

Synonym(s):

BugBuster® Protein Extraction Reagent with Benzonase® Nuclease and rLysozyme solution, Cell Lysis Master Mix

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice

About This Item

NACRES:
NA.32
UNSPSC Code:
41106511
Pricing and availability is not currently available.
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

form

liquid

Quality Level

200

manufacturer/tradename

Novagen®

storage condition

OK to freeze

shipped in

wet ice

storage temp.

2-8°C

General description

The BugBuster® Master Mix enables efficient protein purification and other related applications. It replaces the need for mechanical methods like the French press or sonication. The two available package sizes contain sufficient reagents for extracting proteins from either 20 grams or 100 grams of cell paste.

Application

BugBuster® Master Mix has been used:
  • to resuspend and lyse the cell pellets during protein purification
  • to extract proteins for immunoblotting
  • as a bacterial lysis buffer for the preparation of inclusion bodies


    

Biochem/physiol Actions

BugBuster® Protein Extraction Reagent is specifically designed to gently disrupt the cell wall of E. coli, allowing the release of soluble proteins. This unique formulation utilizes a detergent mix that can effectively perforate the cell wall without denaturing the soluble proteins. By combining BugBuster Reagent, rLysozyme solution, and Benzonase Nuclease, our BugBuster Master Mix enhances protein extraction efficiency and simplifies downstream processing of protein extracts.

Features and Benefits

  • Efficient lysis of Gram-positive and Gram-negative bacteria
  • Maximum recovery of active, soluble protein
  • Capable of cell wall perforation without denaturing soluble protein
  • Useful for the preparation of high purity inclusion bodies
  • Compatible with protease and phosphatase inhibitor cocktail

  • Offers a convenient, fast, and cost-effective solution for releasing the expressed target protein.
  • The Master Mix eliminates the need to dilute or perform separate addition steps.

Legal Information

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
709237092271186
BugBuster® Master Mix

Millipore

71456

BugBuster® Master Mix

BugBuster® (primary amine-free) Extraction Reagent

Millipore

70923

BugBuster® (primary amine-free) Extraction Reagent

BugBuster® HT Protein Extraction Reagent

Millipore

70922

BugBuster® HT Protein Extraction Reagent

YeastBuster Protein Extraction Reagent Efficient extraction of protein from yeast without mechanical disruption and enzymatic lysis

Millipore

71186

YeastBuster Protein Extraction Reagent

storage condition

OK to freeze

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

shipped in

wet ice

shipped in

ambient

shipped in

wet ice

shipped in

wet ice

storage temp.

2-8°C

storage temp.

10-30°C

storage temp.

2-8°C

storage temp.

-

form

liquid

form

liquid

form

liquid

form

liquid

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

-

Storage Class

12 - Non Combustible Liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Related Content

Overnight Express Autoinduction Systems
安定したタンパク質抽出と効率的なサンプル調整に　Buster & Protein Extraction Kit シリーズとタンパク質調整関連製品
Western Blotting Tools

Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. We’ve been helping scientists publish their Western blots for decades, with continued innovation and steadfast technical support. Explore our expanded portfolio of products, including optimized reagents for chemiluminescent and Ḁuorescent Westerns, as well as the SNAP i.d.® system, which reduces blocking, washing and antibody incubation time from hours to minutes.

View All Related Content
Yong Y Peng et al.
Bioengineered, 5(6), 378-385 (2014-12-09)
The collagen like domain Scl2 from Streptococcus pyogenes has been proposed as a potential biomedical material. It is non-cytotoxic and non-immunogenic and can be prepared in good yield in fermentation. The Scl2 collagen domain is about a quarter of the
RT-QuIC assays for prion disease detection and diagnostics
Orru CD, et al.
Journal of biological and chemical chronicles, 185-203 (2017)
Daniel G Isom et al.
Proteins, 79(4), 1034-1047 (2011-03-10)
Protein thermodynamic stability is a fundamental physical characteristic that determines biological function. Furthermore, alteration of thermodynamic stability by macromolecular interactions or biochemical modifications is a powerful tool for assessing the relationship between protein structure, stability, and biological function. High-throughput approaches
View All Related Papers

Global Trade Item Number

SKUGTIN
71456-404055977270150
71456-307790788053260

Questions

Reviews

No rating value

Active Filters