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E6754

Epoxy-activated−Sepharose™ 6B

lyophilized powder

Synonym(s):

Epoxy-activated-Agarose

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:
MFCD00163561
Pricing and availability is not currently available.
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form

lyophilized powder

Quality Level

200

extent of labeling

19-40 μmol per mL

technique(s)

affinity chromatography: suitable

matrix

Sepharose 6B

matrix active group

epoxy

matrix attachment

through epoxy to hydroxyl

matrix spacer

12 atoms

swelling

1 g swells to 3.0-5.0 mL

particle size

45—165 μm

storage temp.

2-8°C

General description

when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether.

Application

Epoxy-activated-Sepharose™ is used in affinity chromatography, protein chromatography, and activated/functionalized matrices. Epoxy-activated-Sepharose™ has been used to obtain a monoclonal antibody against the prokaryotically expressed foot-and-mouth disease virus (FMDV) non-structural protein (NSP) 3B as well as to study B meningococcal disease.

Physical form

Lyophilized powder

Other Notes

Ligand: 1,4-bis(2:3-Epoxypropoxy)butane
Attachment: one epoxy group
Spacer: provides a 12-atom spacer when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether.

Legal Information

Sepharose is a trademark of Cytiva

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This Item
P6649C91426B100
Epoxy-activated−Sepharose™ 6B lyophilized powder

Sigma-Aldrich

E6754

Epoxy-activated−Sepharose™ 6B

Protein A–Sepharose™ 6MB aqueous ethanol suspension

Millipore

P6649

Protein A–Sepharose™ 6MB

Cyanogen bromide-activated-Sepharose™ 4B lyophilized powder

Sigma-Aldrich

C9142

Cyanogen bromide-activated-Sepharose™ 4B

Sepharose™ 6B 6% Beaded Agarose, 45-165 μm (wet), fractionation range 10,000-1,000,000 Da (dextrans)

Sigma-Aldrich

6B100

Sepharose™ 6B

particle size

45—165 μm

particle size

-

particle size

45—165 μm

particle size

45-165 μm (wet)

matrix

Sepharose 6B

matrix

-

matrix

Sepharose 4B

matrix

6% agarose

matrix active group

epoxy

matrix active group

-

matrix active group

cyanate or related structures

matrix active group

-

technique(s)

affinity chromatography: suitable

technique(s)

-

technique(s)

affinity chromatography: suitable

technique(s)

affinity chromatography: suitable

form

lyophilized powder

form

aqueous ethanol suspension

form

lyophilized powder

form

suspension

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000474055
0000474055
0000430142
0000430142
0000351274

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M G Marcu et al.
Journal of the National Cancer Institute, 92(3), 242-248 (2000-02-03)
Heat shock protein 90 (Hsp90) interacts with and stabilizes several oncogenic protein kinases (e.g., p185(erbB2), p60(v-src), and Raf-1) and is required for the stability and dominant-negative function of mutated p53 protein. Two unrelated antibiotics, geldanamycin and radicicol, bind specifically to
Dong Li et al.
Veterinary research communications, 34(5), 445-457 (2010-06-01)
A monoclonal antibody, 3BIgG, against the prokaryotically expressed foot-and-mouth disease virus (FMDV) non-structural protein (NSP) 3B was obtained. The 3BIgG-sepharose conjugant (3BmAb-6BFF) was prepared by adding the purified 3BIgG into epoxy-activated sepharose 6BFF, incubating with the inactivated FMDV, and then
C A O'Brian et al.
Cancer research, 48(13), 3626-3629 (1988-07-01)
We have previously demonstrated that tamoxifen and related triphenylethylene compounds are potent inhibitors of protein kinase C (PKC). The present study demonstrates that PKC binds specifically and reversibly to the antiestrogen N-didesmethyltamoxifen when the drug is coupled to CNBr-activated agarose
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Global Trade Item Number

SKUGTIN
E6754-5G04061833604632

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