This product is provided as a solution with a minimum concentration of 0.50 mg protein/mL Carboxypeptidase B in 0.05 M NaOAc pH 5.0, containing 1.0 M NaCl and 0.01% NaN3. The concentration is lot-specific and reported in the product Certificate of Analysis. The maximum volume, based on the product specification, is 0.2 mL. This product is stored at −20°C and ships on dry ice. Please see the link below to review a sample or lot-specific Certificate of Analysis:
https://www.sigmaaldrich.com/product/sigma/c5233#product-information
C5233
Carboxypeptidase B from human pancreas
50-55 units/mg protein carboxypeptidase B
Synonym(s):
Peptidyl-L-Lysine[L-arginine] hydrolase
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biological source
human pancreas
Quality Segment
form
solution
specific activity
50-55 units/mg protein carboxypeptidase B
impurities
≤0.2% chymotrypsin, ≤0.2% trypsin, ≤1 unit/mg protein carboxypeptidase A
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
SMILES string
S(Cc3ccccc3)C(=O)C(NC(=O)C2N(CCC2)C(=O)C(NC(=O)C(NC(=O)CCC(=O)O)C)C)Cc1ccccc1
InChI
1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
InChI key
TWURVFFNODFJBJ-UHFFFAOYSA-N
Gene Information
human ... CPB1(1360)
General description
Carboxypeptidase B is mapped to human chromosome 3q24.[1] Carboxypeptidase B belongs to A/B subfamily of carboxypeptidases.
Application
Carboxypeptidase B from Sigma has been used as a reference for assaying carboxypeptidase activity in lysed pituitary granules derived from the anterior and intermediate lobes of rat.[2] The enzyme has also been used to digest plasma samples by removing C-terminal basic amino acids, to get a distinct band for each allotype during C4 electrophoresis.[3]
Biochem/physiol Actions
Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 34,000 Da. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro.[4] The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis.
Mutations in the carboxypeptidase B (CPB1) gene is implicated with increased susceptibility to pancreatic cancer development and progression. Elevated levels of CPB1 is associated with low grade breast tumors and lymph node positive grade 1 tumors.
Physical form
Solution in 0.05 M NaOAc pH 5.0 + 1.0 M NaCl + 0.01% NaN3
Other Notes
One unit will hydrolyze 1 μmole of hippuryl-L-arginine per minute at pH 7.7 at 25 °C
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This Item | |||
|---|---|---|---|
| biological source human pancreas | biological source Porcine pancreas | biological source Porcine pancreas | biological source human pancreas |
| Gene Information human ... CPB1(1360) | Gene Information - | Gene Information - | Gene Information human ... CTRB1(1504) |
| specific activity 50-55 units/mg protein carboxypeptidase B | specific activity ≥125 units/mg protein | specific activity ≥100 units/mg protein | specific activity - |
| form solution | form lyophilized powder | form lyophilized | form lyophilized powder |
| UniProt accession no. | UniProt accession no. - | UniProt accession no. - | UniProt accession no. |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
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QC Methods
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C5233-100UG | 04061832700106 |
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I have a question about the Carboxypeptidase B (C-5233). Generally, how much volume of buffer solution (0.05M NaOAc pH 5.0+1.0M NaCl+0.01% NaN3) to add to make the stock? What is the storage temp. for the stock solution after reconstitution? Thank you!
1 answer-
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