This activity of this product is not tested on a lot-to-lot basis. However, historical data indicate a range of 0.28 - 0.40 units per milligram.
A9253
L-Amino Acid Oxidase from Crotalus adamanteus
Type I (dried venom)
Synonym(s):
L-AAO, L-Amino acid: oxygen oxidoreductase (deaminating)
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-564-0
MDL number:
Biological source:
Crotalus adamanteus
Pricing and availability is not currently available.
biological source
Crotalus adamanteus
Quality Level
type
Type I (dried venom), Type I
form
powder
mol wt
~130 kDa
solubility
H2O: soluble 1.0 mg/mL, clear(lit.)
storage temp.
−20°C
General description
L-Amino acid oxidase (LAAO) is a flavoprotein with a molecular weight of 130 kDa. It consists of two different subunits of approximately 70 kDa. Each molecule of holoenzyme has two flavin adenine dinucleotide (FAD) molecules. LAAO is a glycoprotein containing about 2-5% carbohydrate, including sialic acid. optimum pH is approximately 7.5. It occurs in many snake venoms apart from microorganisms and animal tissue, especially in kidney. In the N-terminal region, it has a βαβ domain with glutamic acid residues. LAAO imparts yellow color to venom.
Application
L-Amino Acid Oxidase from Crotalus adamanteus has been used in the quantification of rid protein in imine deaminase activity.
L-amino acid oxidase (LAAO) is used to convert L-amino acids to their corresponding α-keto acids. L-amino acid oxidase, from Sigma, has been used in leucine aminopeptidase (LAP) activity assays.[1] The enzyme has been immobilized and used in an enzymatic flow-injection procedure with chemiluminescence detection for on-site determination of L-alanine.
Biochem/physiol Actions
L-Amino acid oxidase (LAAO) from Crotalus adamanteus requires Mg2+ for its activation. The N-terminal region is essential for FAD binding. The H2O2 generated by LAAO based oxidation reactions lead to prevention of platelet aggregation and induces edema and apoptosis. LAAO activity is inhibited by ethylenediaminetetraacetic acid (EDTA) and phenylmethylsulfonyl fluoride (PMSF).
Preparation Note
Dissolves in water at 1 mg/mL concentration to form a clear solution.
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This Item | |||
|---|---|---|---|
| biological source Crotalus adamanteus | biological source - | biological source - | biological source - |
| form powder | form aqueous suspension | form dried venom | form lyophilized powder |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| solubility H2O: soluble 1.0 mg/mL, clear(lit.) | solubility H2O: soluble 1.0 mg/mL, clear | solubility - | solubility - |
| mol wt ~130 kDa | mol wt ~130 kDa | mol wt - | mol wt ~88 kDa by electrophoresis |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Characterization of L-amino Acid Oxidase Derived from Crotalus adamanteus Venom: Procoagulant and Anticoagulant Activities
Nielsen VG
International Journal of Molecular Sciences, 20(19), 4853-4853 (2019)
The venom-gland transcriptome of the eastern diamondback rattlesnake (Crotalus adamanteus)
Rokyta DR, et al.
BMC Genomics, 13(1), 312-312 (2012)
An enzymatic flow-injection procedure with chemiluminescence detection for on-site determination of L-alanine in synthesis process
Borges EP and Reis BF
Journal of the Brazilian Chemical Society, 16(6), 1226-1232 (2005)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A9253-100MG | 04061833401620 |
| A9253-250MG | 04061832727912 |
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