direct ELISA: 1:30,000 dot blot: 1:2,000 immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 western blot (chemiluminescent): 1:160,000
Immunoglobulin is a glycoprotein, with two heavy chain and two light chain connected by disulfide bond. Immunoglobulin g (IgG) is a major class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2. Rabbit anti-Goat IgG is then conjugated to peroxidase by protein cross linking with 0.2% glutaraldehyde. The conjugate is provided as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT as a preservative.
Immunogen
Purified goat IgG
Application
Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in rabbit has been used in immunoblotting and immunohistochemistry.
Biochem/physiol Actions
Immunoglobulin mainly helps in immune defense. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reaction.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
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