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91A

Leukocyte α-Naphthyl Acetate (Non-Specific Esterase) Kit

Kit formulated with all liquid reagents.

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About This Item

UNSPSC Code:
41116123
NACRES:
NA.47
MDL number:
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Quality Level

form

liquid

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

technique(s)

microbe id | staining: suitable

application(s)

hematology
histology

shipped in

wet ice

storage temp.

2-8°C

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This Item
90A1916911
technique(s)

microbe id | staining: suitable

technique(s)

-

technique(s)

-

technique(s)

-

form

liquid

form

liquid

form

solution

form

solution

application(s)

hematology
histology

application(s)

hematology
histology

application(s)

hematology
histology

application(s)

hematology
histology

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

IVD

for in vitro diagnostic use

IVD

for in vitro diagnostic use

IVD

for in vitro diagnostic use

IVD

for in vitro diagnostic use

Application

Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates. According to the methodology, α-naphthyl acetate is enzymatically hydrolyzed, liberating a free naphthol compound. This then couples with a diazonium compound, forming black colored deposits at sites of non-specific esterase activity. This enzyme is detected primarily in monocytes, macrophages and histocytes, and is normally absent in granulocytes. Lymphocytes may occasionally exhibit enzyme activity. A sodium fluoride inhibition procedure may be run to demonstrate monocyte inhibition. The procedure is run at 37 °C and may be utilized with blood, bone marrow films, tissue-touch preparations, cytocentrifuge preparations and frozen sections. The enzyme does not survive paraffin processing.
Intended for the cytologic demonstration of a-naphthyl acetate esterase in blood, bone marrow films or tissue touch preparations.

Kit Components Only

Product No.
Description

  • TRIZMAL™ 7.6 Concentrate 50 mL

  • Hematoxylin solution gill no. 3 50 mL

Kit Components Also Available Separately

Product No.
Description
SDS

  • 916α-Naphthyl Acetate Solution 10 mLSDS

  • 915Citrate Solution, pH 3.6±0.1 (25 °C), 27 mM 50 mLSDS

  • 917Fast Blue BB Base Solution 10 mLSDS

  • 914Sodium nitrite solution 10 mLSDS

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - STOT SE 1

target_organs

Eyes,Central nervous system

Storage Class

3 - Flammable liquids

flash_point_f

98.1 °F

flash_point_c

36.7 °C

wgk

WGK 2


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S A Kuznetsov et al.
The Journal of cell biology, 153(5), 1133-1140 (2001-05-31)
We report the isolation of adherent, clonogenic, fibroblast-like cells with osteogenic and adipogenic potential from the blood of four mammalian species. These cells phenotypically resemble but are distinguishable from skeletal stem cells found in bone marrow (stromal stem cells, "mesenchymal
Gabrielle S Le Provost et al.
The Journal of investigative dermatology, 135(1), 279-288 (2014-07-23)
During wound healing, excessive inflammation, angiogenesis, and differentiated human dermal fibroblast (HDF ) function contribute to scarring, whereas hyperpigmentation negatively affects scar quality. Over 100 million patients heal with a scar every year. To investigate the role of the beta 2

Global Trade Item Number

SKUGTIN
91A-1KT04061833264577
SAB5201753-100UG04061832160696
SAB5201756-100UG04061832160726
SAB5201767-100UG04061832160832
SAB5201768-100UG04061832160849
SAB5201752-100UG04061832160689
SAB5201754-100UG04061832160702
SAB5201762-100UG04061832160788
SAB5201763-100UG04061832160795
SAB5201764-100UG04061832160801
SAB5201765-100UG04061832160818

Questions

  1. How many tests can be run with the 91A kit (α-Naphthyl Acetate (Non-Specific Esterase)?

    1 answer
    1. The 91A kit is formulated for 10 uses. The Instructions For Use direct the user to prepare 48 mL of staining solution, which is enough to fill a standard Coplin jar. It is possible to stain just 1 slide per run, or up to 10 slides per run for a standard Coplin jar that holds 5 slides, if the slides are placed back to back. The number of slides that can be stained should fall between 10 slides per kit to 100 slides per kit. If the Coplin jar holds more than 5 slides, the number of slides stained per kit would be two times the number of slides the Coplin jar can hold.

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