PROTGA-RO

Protein G Agarose

>98% (HPLC and SDS-PAGE), suspension

• Synonym(s): agarose, protein g
• UNSPSC Code: 41116133
• NACRES: NA.54
• Form: suspension
• Assay: >98% (HPLC and SDS-PAGE)

Properties

• assay: >98% (HPLC and SDS-PAGE)
• form: suspension
• packaging: pkg of 15 mL (05015952001 [settled resin volume]), pkg of 2 mL (11719416001 [settled resin volume]), pkg of 5 mL (11243233001 [settled resin volume])
• manufacturer/tradename: Roche
• storage temp.: 2-8°C

Description

General description

Protein G was initially isolated from G148, a human group G Streptococcal strain. It is a cell wall protein and shows high affinity to IgG (immunoglobulin G). It has a putative molecular weight of 30,000Da. It interacts with all subclasses of human IgG and also binds to mouse, rabbit and goat IgG.[1]

Application

Affinity chromatography using Protein G Agarose, like Protein A Agarose, is the method of choice for purification of IgG from many species. It has been used as a pre-clearing agent during-

• Ubiquitination assay[2][3]
• Immunoprecipitation[2][4]

Biochem/physiol Actions

Protein G is a cell wall protein, isolated from a specific bacterial strain, which has specific binding sites for certain classes of immunoglobulins from different species. Protein G binds nearly all subclasses of IgG, but no other classes of immunoglobulins.
Binding capacity: 20mg human IgG (polyclonal)/ml (the IgG is loaded at pH 7.0, and eluted with 200mM glycine, pH 2.8)
Structure: recombinant Protein G (E. coli, Mr = 22,000) is covalently coupled to crosslinked 4% agarose beads: 2.5mg Protein G (>98% pure)/1ml gel.

Features and Benefits

Contents
Preswollen gel, ready-to-use, 2ml or 5ml gel (settled gel volume) in buffer that contains 14-19% ethanol as a preservative, nonsterile.
Protein G content: 2.5mg/ml preswollen gel

Analysis Note

Purity: >98% (HPLC, SDS-PAGE); entirely free from staphylococcal enterotoxins.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• pictograms: GHS02,GHS07
• signalword: Warning
• pcodes: P210 - P280 - P303 + P361 + P353 - P337 + P313 - P370 + P378 - P403 + P235
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 3
• Storage Class: 3 - Flammable liquids
• wgk: WGK 1
• flash_point_f: 102.2 °F
• flash_point_c: 39 °C
• hcodes: H226,H319