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Digoxigenin-11-UTP

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About This Item

NACRES:

NA.21

UNSPSC Code:

41105500

Assay:

≥85% (HPLC)

Form:

solution

Storage temp.:

−20°C

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Properties

assay

≥85% (HPLC)

form

solution

mol wt

(M_r= 1106.7 (DIG-11-UTP-Li4))

packaging

pkg of 25 μL (11209256910 [10 mM]), pkg of 57 μL (03359247910 [3.5 mM])

manufacturer/tradename

Roche

storage temp.

−20°C

Description

General description

Digoxigenin (DIG) -11-uridine triphosphate (UTP) is provided as a solution of the tetralithium salt in 3.5mM (200nmol) or 10mM (250nmol) concentration. It is used as a substrate for SP6, T3, and T7 RNA polymerases.[1][2] It can replace UTP in the in vitro transcription reaction for DIG labeling of RNA in a ratio of 35:65%.

Application

Digoxigenin-11-UTP has been used for labeling RNA probes in:

in situ hybridization[3][4]
in situ reverse transcriptase (RT)-polymerase chain reaction (PCR)
fluorescence in situ hybridization (FISH)

Biochem/physiol Actions

Linearized template DNA with T7, SP6, or T3 promoter can be in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and digoxigenin-11-UTP, respectively. The labeled RNA can be subsequently detected with the anti-Digoxigenin-AP (alkaline phosphatase), fab fragments, the digoxigenin nucleic acid detection kit, or the digoxigenin luminescent detection kit for nucleic acids.

Analysis Note

Typical analysis: 85% DIG-11-UTP (HPLC, area%)
Function tested with the DIG RNA Labeling Kit.

Formula: C₄₃H₆₁N₄O₂₂P₃Li₄

Other Notes

For life science research only. Not for use in diagnostic procedures.

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This Item	11363905910	DIUTP-RO	DIUTPS-RO
Roche DIGUTP-RO Digoxigenin-11-UTP	Roche 11363905910 Digoxigenin-11-ddUTP	Roche DIUTP-RO Digoxigenin-11-dUTP, alkali-labile	Roche DIUTPS-RO Digoxigenin-11-dUTP, alkali-stable
manufacturer/tradename Roche	manufacturer/tradename Roche	manufacturer/tradename Roche	manufacturer/tradename Roche
form solution	form solution	form solution	form solution
assay ≥85% (HPLC)	assay 85% (HPLC)	assay 85% (HPLC)	assay ≥85% (HPLC)
packaging pkg of 25 μL (11209256910 [10 mM]), pkg of 57 μL (03359247910 [3.5 mM])	packaging pkg of 25 μL (25 nmol; 1mM)	packaging pkg of 125 μL (11573179910 [1 mM]]]), pkg of 25 μL (11573152910 [1 mM])	packaging pkg of 125 μL (11558706910 [1 mM]), pkg of 5 × 125 μL (11570013910 [5x1 mM]), pkg of 25 μL (11093088910 [1 mM])
mol wt (M_r= 1106.7 (DIG-11-UTP-Li4))	mol wt (Molecular weight: M_r = 1074.7 (DIG-11-ddUTP-Li₄))	mol wt -	mol wt M_r 1090.7
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

pictograms

GHS07

signalword

Warning

hcodes

H302

pcodes

P264 - P270 - P301 + P312 + P330 - P501

Hazard Classifications

Acute Tox. 4 Oral

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

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Global Trade Item Number

SKU	GTIN
11209256910	04061837902062
3359247910	04061826742723