H6536

Monoclonal Anti-HIF-1α antibody produced in mouse

~1 mg/mL, clone H1α67, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-Hypoxia Inducible Factor 1, α subunit
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD01633488
• Conjugate: unconjugated
• Clone: H1α67, monoclonal
• Application: IHC (p), IP, WB
• Citations: 22

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: H1α67, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 120 kDa
• species reactivity: rat, sheep, ferret, human, mouse
• concentration: ~1 mg/mL
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500-1:1,000 using antigen retrieval, immunoprecipitation (IP): suitable, western blot: 1:500-1:1,000
• isotype: IgG2b
• UniProt accession no.: Q16665
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... HIF1A(3091) ; mouse ... Hif1a(15251) ; rat ... Hif1a(29560)

Description

General description

Hypoxia-inducible factor-1 (HIF-1) is a 120kD basic helix-loop-helix protein that belongs to the PAS superfamily of transcription factors. It has a crucial role in activating transcription of hypoxia-inducible genes. It can also activate the expression of the gene coding for NIP3; which subsequently induces cell apoptosis under persistent oxygen deprivation. Monoclonal anti-HIF-1α antibody can be used in immunostaining to detect HIF-1α localization. It can also be used in western blotting. Mouse anti-HIF-1α antibody reacts specifically with HIF1 α subunit of sheep, mouse, rat, ferret and human.

Immunogen

peptide corresponding to amino acids 432-528 of the human HIF-1α protein.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Monoclonal anti-HIF-1α antibody (diluted 1: 500) can be used in immunofluorescence confocal microscopy. It can also be used in in immunoblotting, immunoprecipitation and immunohistochemistry.

Physical form

Solution in 0.02 M phosphate buffer containing 0.25 M sodium chloride and 0.1% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable