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A3219

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)

peroxidase substrate, chromogenic, liquid

Synonym(s):

ABTS

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About This Item

UNSPSC Code:
12352204
PubChem Substance ID:
24890730
NACRES:
NA.83
MDL number:
MFCD00214141
Pricing and availability is not currently available.
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Product Name

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), Liquid Substrate System

Quality Level

200

form

liquid

storage temp.

2-8°C

SMILES string

CCN1C(\Sc2cc(ccc12)S(O)(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S(O)(=O)=O

InChI

1S/C18H18N4O6S4/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28)/b19-17-,20-18-

InChI key

ZTOJFFHGPLIVKC-CLFAGFIQSA-N

General description

The product is supplied as a ready-to-use one component substrate for peroxidase consisting 2,2′-azino-bis (3-ethylbenz-thiazoline-6-sulfonic acid) in an acidic buffer. The substrate is colorless before reacting with peroxidase and produces a blue-green color reaction product after reacting with it. 1% sodium dodecyl sulfate (SDS) may be used to stop the reaction during end-point assays. Since this substrate produces a soluble reaction product, it is not recommended for immunoblotting or histochemical applications.

Application

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) has been used as a peroxidase substrate in ELISA experiment. This substrate gives a green color soluble end product.[1][2] It has also been used as a colloidal preparation spread over the standard media plates, to evaluate the lignin oxidation activity. Presence of green coloration of the substrate exhibits microbial laccase activity.[3]

Biochem/physiol Actions

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) is a cation and undergoes oxidation in the presence of hydrogen peroxide. The oxidized ABTS is a blue-green product and is measurable colorimetrically. A combination of ABTS, hydrogen peroxide and myoglobin is a standard system employed for measuring total antioxidant capacity(TAC) from samples. ABTS method is flexible at varying pH and most widely used. ABTS is a substrate for phenol oxidase enzyme.

Physical form

Ready-to-use.

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This Item
A1888A9941194434
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Liquid Substrate System

Sigma-Aldrich

A3219

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt ≥98% (HPLC)

Sigma-Aldrich

A1888

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt tablet, 10 mg substrate per tablet

Sigma-Aldrich

A9941

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

ABTS™ Chromophore

Sigma-Aldrich

194434

ABTS™ Chromophore

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

form

liquid

form

powder

form

tablet

form

solution

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

2-8°C

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
MKCZ9807
MKCW8098
MKCV7001
MKCT8008
MKCW3025

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Related Content

A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation
Erel O
Clinical Biochemistry, 37(4), 277-285 (2004)
Randall L Davis et al.
Journal of neuroimmunology, 186(1-2), 141-149 (2007-05-04)
Emerging evidence indicates that neuroinflammatory responses in astroglia, including chemokine expression, are altered by opioids. Astroglial chemokines, such as CXCL10, are instrumental in response to many neuropathological insults. Opioid mediated disruption of astroglial CXCL10 expression may be detrimental in opioid
Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis
Shalaby EA and Shanab SMM
Indian Journal of Geo-Marine Sciences, 42(5), 556-564 (2013)
View All Related Papers

Global Trade Item Number

SKUGTIN
A3219-100ML04061838251572

Questions

1–9 of 9 Questions  

  1. what is the molarity of this solution?

    1 answer

    1. This product is a 1.8 mM solution of ABTS

      Helpful?

  2. Can this product be used for antioxidant assay?

    1 answer

    1. This product has not been specifically tested for use in antioxidant assays. However, it has been used successfully as such in several publications. Please see the link below to review one example:
      https://www.sciencedirect.com/science/article/pii/S0009912003002315?via%3Dihub

      Helpful?

  3. What is the approximate concentration of H2O2 in the ready-to-use A3219 solution?

    1 answer

    1. This product is no longer tested for hydrogen peroxide concentration. The previous specification for H2O2 was </= 0.03%. Current lots are expected to be within this range.

      Helpful?

  4. What is the molecular weight and density?

    1 answer

    1. This product is a 1.8 mM solution of ABTS, the density is not determined. The solution is prepared from a diammonium salt form with a molecular weight of 548.60.

      Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/338/745/a3219dat.pdf

      Helpful?

  5. ABTS ready to use를 사용하여 항산화 실험을 하는경우 기존의 방법에 적용하면 어떤 단계가 생략이 되는건지 자세한 실험 과정을 알고 싶습니다

    1 answer

    1. The only protocol currently available on the website is this data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/338/745/a3219dat.pdf.

      It may be helpful to also view the "Peer-Reviewed Papers" related to this product: https://www.sigmaaldrich.com/search/a3219?focus=papers&page=1&perpage=30&sort=relevance&term=a3219&type=citation_search.

      Helpful?

  6. When using Product A3219, 2,2´-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), at what wavelength should the product of the reaction be measured?

    1 answer

    1. The product should be measured at 405 nm. For end point assays, the reaction may be stopped with 1% (w/v) SDS and also read at 405 nm.

      Helpful?

  7. What is the concentration of Product A3219, 2,2´-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)?

    1 answer

    1. The concentration of the ABTS is approximately 1.8 mM.

      Helpful?

  8. Should Product A3219, 2,2´-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), be diluted prior to use?

    1 answer

    1. No, this product is offered as a ready-to-use (1×) solution.

      Helpful?

  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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