04-263

Anti-Ubiquitinylated proteins Antibody, clone FK2

clone FK2, Upstate^®, from mouse

• Synonym(s): Anti-ubiquitin antibody, FK2 ubiquitin antibody
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: FK2, monoclonal
• Technique(s): ELISA: suitable, immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• Application: ELISA, IF, IP, WB
• Citations: 165

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: FK2, monoclonal
• species reactivity (predicted by homology): all
• manufacturer/tradename: Upstate^®
• technique(s): ELISA: suitable, immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG1
• shipped in: dry ice
• target post-translational modification: unmodified

Description

General description

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.

Immunogen

Poly-ubiquitinylated-lysozyme.

Application

Research Category
Protein Trafficking

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

This Anti-Ubiquitinylated proteins Antibody, clone FK2 has been published and validated for use in ELISA, Immunofluorescence (IF), Immunoprecipitation (IP) and Western Blotting (WB) for the detection of Ubiquitinylated proteins.

Biochem/physiol Actions

Recognizes mono- and poly ubiquitinylated proteins, but not free ubiquitin.

Physical form

100 µL of purified immunoglobulin in PBS, pH 7.4 containing 50% glycerol and 0.09% sodium azide. Dilute to working strength with phosphate buffered saline pH 7.2-7.4.

Format: Purified

Preparation Note

Stable for 1 year at -20°C from date of shipment for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable