Long-term storage of silica-based columns is best in 100% acetonitrile. Columns may be safely stored for short periods (up to 3 or 4 days) in most common mobile phases. However, when using buffers, it is best to remove the salts to protect both the column and the HPLC equipment by first flushing the column with the same mobile phase without the buffer (e.g., when using 90/10 ACN/buffer, flush the column with 90/10 ACN/H2O) to eliminate any concern about salt precipitation or corrosion from the salts then flush the column with 100% acetonitrile for storage.Before storing the column, the end-fittings should be tightly sealed with the endplugs that came with the column to prevent the packing from drying.
Kluczowe dokumenty
53939-U
Ascentis® Express 90 Å HILIC (2.7 μm) HPLC Columns
L × I.D. 10 cm × 2.1 mm, HPLC Column
Synonim(y):
Core-shell (SPP) Fused Core Si HPLC column
Wybierz wielkość
3730,00 zł
Wybierz wielkość
About This Item
3730,00 zł
Polecane produkty
Nazwa produktu
Ascentis® Express HILIC, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 10 cm × 2.1 mm
Materiały
stainless steel column
Poziom jakości
agency
suitable for USP L3
linia produktu
Ascentis®
Właściwości
endcapped: no
producent / nazwa handlowa
Ascentis®
opakowanie
1 ea of
Parametry
≤100 °C temp. range
600 bar max. pressure (9000 psi)
metody
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
dł. × śr. wewn.
10 cm × 2.1 mm
powierzchnia
135 m2/g
zanieczyszczenia
<5 ppm metals
Matryca
Fused-Core particle platform
superficially porous particle
grupa aktywna macierzy
silica phase
wielkość cząstki
2.7 μm
wielkość porów
90 Å
pH robocze
1-8
Zastosowanie
food and beverages
metoda separacji
hydrophilic interaction (HILIC)
normal phase
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Opis ogólny
Visit the Ascentis Express home page for more information on this new column technology.
Informacje prawne
Zastosowanie
kartridż do prekolumny
kolumna analityczna
produkt powiązany
wymagane, ale niedostarczone
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Certyfikaty analizy (CoA)
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Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Produkty
A significantly improved HPLC-fluorescence method for DMB-NANA and -NGNA, and application of this method to compare 2 candidate biosimilar therapeutic proteins to their respective RMs.
W tym artykule podkreślono wpływ efektów matrycy próbki na odpowiedź LC/MS i omówiono dwa nowe podejścia do ich zmniejszenia.
This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.
This article evaluates different phase chemistries for stationary phases in Hydrophilic Interaction Liquid Chromatography (HILIC) and uses multivariate analysis for classification based on chemical modification.
Protokoły
We offer the tools for the analysis of the metabolites; including certified reference standards, enzymes, substrates, and chromatographic products.
Oferujemy narzędzia do analizy metabolitów, w tym certyfikowane wzorce referencyjne, enzymy, substraty i produkty chromatograficzne.
Analytical standards for drug analysis including heroin, cocaine, amphetamine, and methylenedioxymethamphetamine.
Chromatograms
application for HPLCapplication for HPLCapplication for HPLCapplication for HPLCPokaż więcej-
How should I store the Ascentis Express HILIC column?
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In HILIC separations, what happens if the sample is an aqueous matrix? Does it always have a negative effect?
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Yes, it would be highly preferential (especially in this case where you want partitioning to dominate) to inject in high organic. That said, you can 'get away' with it if the injection volume can be kept small - much like we can inject low volumes of stronger solvents in RP mode, if needed. What you will want to do to minimize impact is to get as much retention on the analytes of interest as you can, this helps give the sample solvent some time to dissipate and negate the effects.
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Can peptide or protein samples be analyzed using HILIC columns?
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Polar peptides are quite amenable to HILIC separations; however, our experience with larger peptides has been only minimally successful - mainly due to solubility issues. Proteins are even more difficult due to the same issue. An additional problem with proteins is that they are often multiply charged. When IEX is performed on multiply charged analytes, you often get what is referred to as a rolling effect where the analyte interacts with ionic sites on the surface in many different ways as it 'rolls' down the column; this produces broad and misshapen peaks.
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What flow rate should I use with Ascentis® Express HPLC Columns?
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Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
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When you recommend only changing one parameter at a time, does this also refer to the total ionic strength?
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If you change organic, try to keep the overall buffer concentration (and all other parameters, for that matter) constant. There are times when you will want to change both and perhaps pH/temp/etc. simultaneously, but that drastically complicates the system and thus should be avoided, if possible.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can I use Ascentis Express on any type of HPLC system?
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Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems. Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
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Why is it recommended to run isocratically for HILIC methods?
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When running in HILIC mode, both isocratic and gradient practices result in instability. If you keep the re-equilibration times constant, gradient should not be a problem, but changing this parameter can have a significant impact. It is not so much that it is bad as it is different than we are used to in reversed phase. Usually, we assume that once equilibrated (5, 10, 15 min, etc.), we can leave the system for any time period and come back to the same results. This does not appear to be the case in our studies of HILIC. Knowing that the re-equilibration time has an impact, you should get in the habit of making several injections with known re-equilibration times prior to making any development decisions. To get around this, isocratic runs are recommended. Attached are two posters; the first was presented at HPLC 2013 (Amsterdam) and the second was presented at Balaton Symposium on High Performance Separation Methods 2013 (Hungary). Both show 'reproducibility' at any set re-equilibration time is good but both show that if you change the re-equilibration time; then retention, peak shape and selectivity can change especially where ionic interactions are prevalent.
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Is there anything special I need to do to my HPLC system to use Ascentis Express?
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Nothing special is required to use Ascentis Express HPLC columns. To obtain the full benefits of Ascentis Express, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102)
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How does the flow rate influence the water layer on the column?
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We are not aware of any systematic studies with respect to the impact of flow rate on HILIC separations. Our concern would be that as you move to higher flow rates, you might observe peak shape issues due to the slow kinetics of IEX and adsorption mechanisms. If the retention mechanisms for a given system are partition dominated, this should be of less concern. It will be a case by case cause and effect.
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