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H8889

Histopaque®-1077 Hybri-Max

liquid, sterile-filtered, BioReagent, suitable for hybridoma

Synonim(y):

Histopaque 1077

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Do Państwa/SKUDostępnośćCena netto
100 mL
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500,00 zł
500 mL
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
1660,00 zł

Informacje o tej pozycji

NACRES:
NA.75
UNSPSC Code:
12352201

500,00 zł


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sterility

sterile-filtered

Quality Segment

product line

BioReagent

form

liquid

technique(s)

cell culture | hybridoma: suitable, single cell analysis: suitable

impurities

endotoxin, tested

density

1.077 at 25 °C (relative density)(lit.)

storage temp.

2-8°C

General description

Histopaque®-1077 is a solution comprising polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm3. This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell-mediated lympholysis and human lymphocyte antigen (HLA)2 typing. It may also be employed as the initial isolation step before the enumeration of T-, B-, and null lymphocytes. It is used to create a density medium for the purification of lymphocytes and other mononuclear cells.

Application

Histopaque®-1077 Hybri-Max has been used to isolate:
  • peripheral blood mononuclear cells (PBMCs) from heparinized blood by gradient centrifugation
  • immune cells (lymphocytes) from peripheral blood
  • PBMCs from blood

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
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Ta pozycja
1077110831A6929
description

liquid, sterile-filtered, BioReagent, suitable for hybridoma

description

sterile-filtered, density: 1.077

description

sterile-filtered, density: 1.083

description

sterile-filtered, density: 1.077 g/mL

technique(s)

single cell analysis: suitable, cell culture | hybridoma: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

density

1.077 at 25 °C (relative density)(lit.)

density

1.077

density

1.083

density

1.077 g/mL

form

liquid

form

liquid

form

liquid

form

solution

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

aseptically filled

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

400

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C


pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Klasa składowania

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter



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Questions

1–3 of 3 Questions  
  1. How many plasma can be processed through on 100 mL pack. And what all items will come with pack? detailed product information is required.

    1 answer
    1. The amount of plasma that can be processed using a 100 mL pack of Histopaque®-1077 depends on the specific protocol and the volume of blood being processed. Histopaque®-1077 is a solution comprising polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm3. The detailed protocol can be found here:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/284/292/h8889pis.pdf

      Helpful?

  2. Is there a product in your inventory that is comparable to the Corning product 25-072-CV?

    1 answer
    1. Upon comparison of the formulation and product description, the comparable product from MilliporeSigma is identified as product number H8889. Both products, 25-072 and H8889, have a density of 1.077 g/ml and share the same formulation components, including Diatrizoic acid at 96.219 and Polysucrose 400 at 61.363.

      Helpful?

  3. What is the formulation for H8889 Histopaque-1077 Hybri-Max?

    1 answer
    1. The formulation for H8889 Histopaque-1077 Hybri-Max consists of 5.7% polysucrose 400 and 9% sodium diatrizoate.

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