Yes. 20 grams of F2637 in 100ml of water should be the equivalent to the 20% Ficoll solution offered as catalog number F5415. It should however be noted that there is a density specification for product F5412. The density has to be within a range of 1.055 to 1.075 g/ml. Lot specific results will be reported on lot specific Certificates of Analysis.
Przejdź do
F5415
Ficoll® solution
Type 400, 20% in H2O
Synonim(y):
Ready-to-use density gradient media
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 25 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 416,00 zł | |
| 50 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 700,00 zł |
Informacje o tej pozycji
416,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócsterility
0.2 μm filtered
Quality Level
type
Type 400
form
liquid
concentration
20% in H2O
technique(s)
titration: suitable
color
clear colorless
solubility
water: soluble
density
1.055-1.075 g/mL
application(s)
hematology
histology
storage temp.
2-8°C
General description
Ficoll® Solution is a highly branched polymer of sucrose, also known as polydisperse mixtures of dextran. Ficoll® molecules are inert and not re-absorbed by proximal tubules. The structure is open and deformable, and not a rigid sphere thereby becoming a preferred choice in “crowding” experiments.
Used in electrophoresis and as a hapten carrier. Most commonly used to prepare density gradients.
Application
Ficoll® has been used in
- Isolation of cord blood mononuclear cells
- Isolation of CD133 negative cells
- Isolation and expansion of circulating endothelial progenitor cells.
Other Notes
A nonionic synthetic polymer of sucrose.
Legal Information
Ficoll is a registered trademark of Cytiva
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Ta pozycja | |||
|---|---|---|---|
| technique(s) titration: suitable | technique(s) tissue culture: suitable | technique(s) - | technique(s) - |
| color clear colorless | color white to off-white | color - | color white |
| concentration 20% in H2O | concentration - | concentration - | concentration - |
| solubility water: soluble | solubility H2O: 100 mg/mL, clear to slightly hazy, colorless to faintly yellow | solubility - | solubility water: soluble |
| application(s) hematology | application(s) - | application(s) - | application(s) - |
| form liquid | form powder, spray-dried | form lyophilized powder | form solid |
Klasa składowania
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Protokoły
Protokoły hodowli hematopoetycznych komórek macierzystych do izolacji, ekspansji i różnicowania komórek CD34+, w tym testy CFU.
Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.
Effect of mononuclear cells on morphometric of kidney parameters in hyperglycemic rat.
Salmani F, et al.
Annals of Experimental Biology, 2(3), 1-7 (2014)
Junchao Xing et al.
Tissue engineering. Part C, Methods, 25(3), 137-147 (2019-02-09)
Patient-specific individual tissue-engineered bones (iTEBs) have been recognized as a promising strategy for treating large bone defects. However, current construction protocols of iTEBs vary between lots and lack standardization and quality control, hampering further research and application. This study was
Circulation enrichment of functional endothelial progenitor cells by infantile phototherapy.
Siavashi V, et al.
Journal of Cellular Biochemistry, 118(2), 330-340 (2017)
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| F5415-25ML | 04061833618233 |
| F5415-50ML | 04061833618240 |
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I have used Ficoll400 (F2637) from powder and consider to switch to your 20% solution. Is this product the same as dissolving e.g. 20 g of F2647 in water for a final volume of 100 mL?
1 answer-
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What are the differences between Ficoll, Ficoll Paque Plus, Ficoll Paque Premium, Histopaque and the different products with different densities?
1 answer-
1. Ficoll-Paque PREMIUM products - Sterile, ready-to-use density gradient media for the preparation of mononuclear cells - Based on Ficoll-Paque PLUS - Used for isolating high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood
2. Regulatory Status - Neither Ficoll-Paque Plus nor Ficoll-Paque Premium products are approved for In Vitro Diagnostic Use - For clinical studies, a GMP grade product (Ficoll-Paque Premium) is preferred over a product produced under ISO 9001 - Both products are sterile and low in endotoxins
3. Differences between Ficoll and Histopaque - Ficoll and Histopaque are essentially the same formulation - Ficoll is a trademark name for polysucrose 400, and Paque is a trademark name for sodium diatrizoate - Both can be used to separate various cell types based on different densities and species
4.Cell Separation Using Ficoll and Histopaque - Different densities are used for different cell types and sometimes the same cell types, but from different species - Ficoll and Histopaque can be used to separate a variety of cell types based on different densities and speciesHelpful?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can more Ficoll be added to Product F5415, Ficoll® solution 20%, to increase the density to 1.077 g/mL?
1 answer-
This is not recommended. Ficoll has a molecular weight in the range of 400,000. Even 20% solutions of such high molecular weight products have extremely low osmolality. Without the addition of other lower molecular weight chemical, solutions of 20%+ of Ficoll are considered extremely hypotonic. Cells exposed to hypotonic solutions would not maintain viability and are prone to bursting.
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Is Product F5415, Ficoll® solution 20% suitable for the separation of mononuclear cells without further dilution?
1 answer-
No. The 20% solution of Ficoll has a density of approximately 1.066 to 1.068 g/mL. Typically products such as Histopaque 1077-1 or Ficoll Paque have a density of approximately 1.076 to 1.078 g/mL. Such ready to use solutions are also osmotically balanced by the addition of a lower molecular weight chemical. Properly balanced solutions will typically have an osmolality in the range of approximately 280-300 mOsm/Kg. If a ready made product is required for the separation of mononuclear cells, products Histopaque 1077-1 or H8889 should be utilized.
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Is there a Product Information Sheet for Product F5415, Ficoll® solution 20%?
1 answer-
No. However there is a Product Information Sheet for product F4375. This product information sheet gives general information regarding Ficoll and possible uses. If mononuclear cells are to be separated, a better choice of products is Histopaque 1077-1, H8889 or one of the prefilled Accuspin tubes.
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Is Product F5415, Ficoll® solution 20%, sterile?
1 answer-
No claims are made for sterility for F5415. If a sterile product is required for cell culture use, product F8636 has been gamma irradiated and is considered a sterile product. Product F8636 is a powder and does not come as a sterile solution.
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What is the difference between Product F5415, Ficoll® solution 20%, and the polysucrose type 400 used in the Histopaque 1077-1?
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Ficoll is a registered trademark. The trademark was formerly held by Pharmacia, but today Amersham Biosciences/GE Healthcare hold the trademark. Since Sigma Aldrich uses polysucrose type 400 in the preparation of the various Histopaque products, the name polysucrose 400 is utilized.
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