to deplete abundant plasma proteins for two-dimensional electrophoresis[4]
Biochem/physiol Actions
ProteoPrep blue albumin depletion kit is designed to specifically remove albumin and IgG from 25 samples of human serum (25μL to 100μL) in preparation for two-dimensional electrophoresis (2DE). Albumin-depleted serum samples generated by this kit are in urea rather than salt-based buffers with little dilution, eliminating the need to precipitate the sample prior to performing 2DE.
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ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany
Methods in molecular biology (Clifton, N.J.), 818, 81-96 (2011-11-16)
The development of a sensitive, specific, and non-invasive approach for cancer detection will facilitate early detection and, hence, improve the outcome of individuals with known cancer predispositions. Proteomic profiling of blood emerges to be a logical choice of such non-invasive
Sepsis is defined as an organ dysfunction syndrome and it has high mortality worldwide. This study analysed the proteome of serum from patients with sepsis to characterize the pathological mechanism and pathways involved in sepsis. A total of 59 patients
Chronic HBV infection (CHB) can lead to acute-on-chronic liver failure (HBV-ACLF) characterized by high mortality. This study aimed to reveal ACLF-related proteomic alterations, from which protein based diagnostic and prognostic scores for HBV-ACLF were developed. Methods: Ten healthy controls, 16
Annals of translational medicine, 9(3), 255-255 (2021-03-13)
Klippel-Feil syndrome (KFS) represents the rare and complex deformity characterized by congenital defects in the formation or segmentation of the cervical vertebrae. There is a wide gap in understanding the detailed mechanisms of KFS because of its rarity, heterogeneity, small
The Journal of clinical investigation, 130(4), 2069-2080 (2020-03-17)
BACKGROUNDHBV-related acute-on-chronic liver failure (HBV-ACLF) is hallmarked by high short-term mortality rates, calling for accurate prognostic biomarkers for initial risk stratification.METHODSThree tandem mass tag-labeled (TMT-labeled) quantitative proteomic studies were performed on 10 patients with HBV-related acute hepatic decompensation and on
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