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FLAA

Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit

for ATP quantitation

Sinónimos:

ATP Bioluminescence Assay, ATP Determination Kit, Luminescent ATP Detection kit

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EC Number:
213-579-1
NACRES:
NA.26
eCl@ss:
32160414
UNSPSC Code:
41116133
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Quality Level

200

storage temp.

−20°C

General description

The Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit may be employed for the quantitative bioluminescent determination of ATP in samples. ATP is consumed and light is emitted when luciferase catalyzes the oxidation of D-luciferin. When ATP is the limiting reagent, the light emitted is proportional to the ATP present in the sample.

Application

Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit has also been used in the quantification of ATP in 3D matrixes of human neurons,[1] hepatic cells with ischemia,[2] various bacterial cultures[3] and lysosomes.[4]

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Este artículo
FLASCA8937A2767
Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit for ATP quantitation

Sigma-Aldrich

FLAA

Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit

Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit for cellular ATP determination

Sigma-Aldrich

FLASC

Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit

Adenosine 5′-triphosphate dipotassium salt hydrate from microbial, ≥92% (HPLC)

Sigma-Aldrich

A8937

Adenosine 5′-triphosphate dipotassium salt hydrate

Adenosine 5′-triphosphate–Agarose lyophilized powder

Sigma-Aldrich

A2767

Adenosine 5′-triphosphate–Agarose

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS & Precios
  • FLAAB
    Adenosine 5′-triphosphate (ATP) assay mix dilution buffer, lyophilized powder mL/vial
    SDS
  • FLAAM
    Adenosine 5′-triphosphate (ATP) assay mix, lyophilized powder mL/vial
    SDS
  • FLAAS
    Adenosine 5′-triphosphate (ATP) disodium salt hydrate, vial of ~1 mg ATP mL/vial
    SDS

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H318

Hazard Classifications

Eye Dam. 1

wgk

WGK 3

Clase de almacenamiento

11 - Combustible Solids

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Certificados de análisis (COA)

Lot/Batch Number
0000502396
0000502395
0000502396
0000502395
0000502399

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Número de artículo de comercio global

SKUGTIN
FLAA-1KT04061835556915

Questions

1–8 of 8 Questions  

  1. How many times can one do with Product FLAAS, ATP Bioluminescent Assay Kit (one vial of FLAAS) when standard range between 20uM~0.002uM?

    1 answer

    1. The number of times that one can use FLAAS ATP Assay Kit is dependent upon the sample. Frequently, serial dilutions of the sample, as well as the kit reagents, are required. If no dilutions were required the ATP Assay MIx is reconstituted to 5 mL and added to the sample at 0.1 mL. The dilution factor will need to be determined by the end-user. Please note, FLAAS is stable for at least 24 hours at 2–8 °C, or over two weeks if stored frozen at –20 °C. Working concentrations of the serially diluted standard are stable for up to 8 hours when stored in ice.

      Please view the product data sheet for more information, including dilution factor details:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/308/573/flaabul.pdf

      Helpful?

  2. How does the kit perform on samples with pH 7.3-7.4 (physiological range)? It recommends adjustment of sample pH to 7.8 which is not always possible.

    1 answer

    1. It is important to use correct pH-value. The luciferase reaction has its pH-optimum in the range of 7.75. Samples having extreme high pH-values or samples of a different pH containing high buffer concentrations may influence the light reaction in an unpredictable way. To avoid issues, correct the pH to a value between 7.6 and 8.0 or dilute samples in Tricine Buffer of the appropriate pH.

      Helpful?

  3. Can Product FLAA, ATP Bioluminescent Assay Kit assay mix be used on cells and tissues?

    1 answer

    1. This reagent measures only soluble ATP. Cells must be washed and then lysed to obtain a soluble ATP extract. Tissues must be homogenized and clarified to provide an extract. The supernatant solution can then be used for assay.

      Helpful?

  4. What is the sensitivity of Product FLAA, ATP Bioluminescent Assay Kit the assay mix?

    1 answer

    1. With FLAAM diluted 1:5, one can easily measure 1 pmol ATP in 100 μl (10 nM) with 50 μl of the reagent.

      Helpful?

  5. What  is the stability of the Product FLAA, ATP Bioluminescent Assay Kit ATP stock solution?

    1 answer

    1. The ATP standard stock solution (FLAAS) is stable for at least 24 hours at 0-5°C, or for over two weeks if stored frozen at -20°C.

      Helpful?

  6. How many reactions can one do with Product FLAA, ATP Bioluminescent Assay Kit (one vial of FLAAM)?

    1 answer

    1. When one reconstitutes the vial to 5 ml, as directed, one can perform 50 assays using 100 ul per test. If one dilutes 1:5 one can get 250 tests per kit.

      Helpful?

  7. How can one check for inhibitors in the samples when using Product FLAA, ATP Bioluminescent Assay Kit?

    1 answer

    1. One must add an amount of ATP from a standard solution equivalent to the ATP already found in the sample. If the signal is doubled, the absence of an inhibitor is indicated.

      Helpful?

  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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