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D2902

Sigma-Aldrich

DMEM - low glucose

With ʟ-glutamine and sodium pyruvate, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Synonym(s):

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.71

product name

Dulbecco′s Modified Eagle′s Medium - low glucose, With 1000 mg/L glucose and L-glutamine, without sodium bicarbonate and phenol red, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

H2O: soluble 10 g/L, clear

components

glucose: low
NaHCO3: no
phenol red: no
HEPES: no
L-glutamine: yes
sodium pyruvate: yes

shipped in

ambient

storage temp.

2-8°C

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Application

Dulbecco′s Modified Eagle′s Medium - low glucose is used in cell line culture (used in medium for the growth of cells) and for their maintenance. It is also used for tissue culture.

Quantity

Formulated to contain 10.0 grams of powder per liter of medium.

Reconstitution

Supplement with 3.7 g/L sodium bicarbonate.

Other Notes

Investigators who work with stem cells or cell clones frequently prefer media without phenol red.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1


Certificates of Analysis (COA)

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P C Zamecnik et al.
Proceedings of the National Academy of Sciences of the United States of America, 75(1), 280-284 (1978-01-01)
The tridecamer d(A-A-T-G-G-T-A-A-A-A-T-G-G), which is complementary to 13 nucleotides of the 3'- and 5'-reiterated terminal sequences of Rous sarcoma virus 35S RNA, was added to chick embryo fibroblast tissue cultures infected with Rous sarcoma virus. Inhibition of virus production resulted.
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Prior studies have suggested that the interactions occurring between VEGFR2 extracellular domains in the absence of ligand are complex. Here we seek novel insights into these interactions, and into the role of the different Ig-like domains (D1 through D7) in
Michael D Paul et al.
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Receptor tyrosine kinases (RTKs) are single-pass membrane proteins that control vital cell processes such as cell growth, survival, and differentiation. There is a growing body of evidence that RTKs from different subfamilies can interact and that these diverse interactions can
Maria Guadalupe Martinez et al.
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Alphaviruses are highly organized enveloped RNA viruses with an internal nucleocapsid surrounded by a membrane containing the E2 and E1 transmembrane proteins. Alphavirus budding takes place at the plasma membrane and requires the interaction of the cytoplasmic domain of E2

Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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