STR1

Enhanced Avian First Strand Synthesis Kit

Components for cDNA synthesis with enhanced AMV reverse transcriptase

• Synonym(s): Reverse Transcriptase cDNA synthesis kit, eAMV-RT
• UNSPSC Code: 12352200
• NACRES: NA.55

Properties

• Quality Level: 200
• usage: sufficient for 50 reactions; kit sufficient for 50 reactions
• feature: dNTPs included; hotstart: no
• technique(s): RT-PCR: suitable
• color: colorless
• input: purified RNA
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Enhanced Avian First Strand Synthesis Kit utilizes a highly purified avian myeloblastosis virus reverse transcriptase (eAMV-RT) that offers superior performance in comparison to standard AMV-RT or standard Moloney murine leukemia virus reverse transcriptase (MMLV-RT). This exceptionally robust eAMV-RT has an enhanced ability to transcribe through difficult secondary structure at elevated temperatures (up to 65 °C) making it the ideal enzyme for producing high quality full-length cDNA from total RNA or poly(A)⁺ RNA.

Sigma′s Enhanced Avian RT First Strand Synthesis Kit also provides random nonamers (9-mers) and anchored oligo (dT)23 primers since gene-specific primers may not always be useful or possible. The anchored oligo has 23 thymidine residues and one G, C, or A residue (the anchor). The anchor ensures that the oligo (dT) primer binds at the very beginning of the message and that there is not a long region of the unusable sequence. With this kit, a dependable cDNA is generated that can be used for various downstream applications, including PCR.

Application

Enhanced Avian First Strand Synthesis Kit has been used for cDNA synthesis, which is intended for use in a real-time reverse transcription-polymerase chain reaction (RT-PCR).

Unit Definition

One unit incorporates one nanomole of TMP into TCA-precipitable material in 10 minutes using polyadenylic acid as template and oligo(dT)_12-18 as primer.

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Kit Components Only

• Enhanced Avian Reverse Transcriptase 1000 U

Kit Components Also Available Separately

• Deoxynucleotide mix 50 μL
• O4387 Anchored oligo (dT)₂₃ 100 μL
• R7647 Random nonamers 100 μL
• O4387 Ribonuclease inhibitor 50 μL
• W1754 PCR grade water 1.5 mL

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable