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M5519

Murashige and Skoog Basal Medium

powder, suitable for plant cell culture

Synonym(s):

MS0 Medium, MSO Medium, MS Basal Medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.72
MDL number:
MFCD00240976
Pricing and availability is not currently available.
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form

powder

Quality Segment

200

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

General description

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Application

Murashige and Skoog medium has been used in plant tissue culture medium for plant transformation study.[1][2]

Preparation Note

Formulated to contain 4.4 grams of powder per liter of medium.
Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog medium (M5519) contains the micronutrients and vitamins of the original classic formulation. It can be supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.
With the macro- and micronutrients, and vitamins as described by Murashige and Skoog (1962).

Media Formulation

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Sigma-Aldrich

Sigma-Aldrich

M5519

Murashige and Skoog Basal Medium

Sigma-Aldrich

Sigma-Aldrich

M0404

Murashige and Skoog Basal Medium

Murashige and Skoog Shoot Multiplication Medium A powder, suitable for plant cell culture

Sigma-Aldrich

M7024

Murashige and Skoog Shoot Multiplication Medium A

Murashige and Skoog Basal Medium suitable for plant cell culture, with sucrose and agar

Sigma-Aldrich

M9274

Murashige and Skoog Basal Medium

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

form

powder

form

powder

form

powder

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

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pictograms

Flame over circleExclamation mark
GHS03,GHS07

signalword

Warning

hcodes

H272,H319

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 3

Storage Class

5.1B - Oxidizing hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000546778
0000546778
0000457359
0000457359
0000423246

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Joan E Krochko et al.
Methods in molecular biology (Clifton, N.J.), 773, 113-134 (2011-09-08)
In vitro assays for cytochrome P450 enzymes developed from plant-derived microsomal extracts have not been used extensively for the characterization and quantification of enzyme activities in plant tissues. We describe here an in vitro assay for abscisic acid (ABA) 8'-hydroxylase
Ajay Kumar et al.
BMC genomics, 13, 597-597 (2012-11-07)
Development of a high quality reference sequence is a daunting task in crops like wheat with large (~17Gb), highly repetitive (>80%) and polyploid genome. To achieve complete sequence assembly of such genomes, development of a high quality physical map is
Youjun Zhang et al.
Plant communications, 1(5), 100028-100028 (2020-12-29)
Although the use of stable transformation technology has led to great insight into gene function, its application in high-throughput studies remains arduous. Agro-infiltration have been widely used in species such as Nicotiana benthamiana for the rapid detection of gene expression and
View All Related Papers

Global Trade Item Number

SKUGTIN
M5519-BG04061824042252
M5519-10L04061838259745
M5519-50K338204061832957395
M5519-1L04061835568857
M5519-50L04061838259752
M5519-50.1K04061832957388

Questions

1–10 of 11 Questions  

  1. How do I prepare this medium?

    1 answer

    1. The preparation instructions for this product are as follows:
      1. Using a container twice the size of the desired final volume, measure out ~90% of the required final volume of tissue culture grade water (e.g., Catalog Number W3500). Example: 900 ml for a final volume of 1000 ml.
      2. While stirring, add the powdered product.
      3. Rinse the original container with a small volume of tissue culture grade water to remove traces of the powder. Add to the solution in step 2.
      4. Add desired supplements (e.g., sucrose, gelling agent, auxins, cytokinins).
      Add heat labile constituents after autoclaving.
      5. While stirring, adjust to the desired pH (e.g., 5.7±0.1) using KOH, NaOH, or HCl.
      6. Add additional tissue culture grade water to bring the medium to the final volume.
      7. If a gelling agent is used, heat the solution to clarity while stirring.
      8. Dispense the medium into culture vessels before or after autoclaving according to the application.
      Add heat labile constituents after autoclaving.
      9. Sterilize the medium in a validated autoclave at 1 kg/cm2 (15 psi). The medium should attain a temperature of 121 °C for at least 15 minutes.

      Please see the link below to review this and additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf

      Helpful?

  2. What is the difference between Product M5519, Murashige and Skoog (MS) Basal Medium and M5524, Murashige and Skoog Basal Salt Mixture?

    1 answer

    1. Product M5524 is the Murashige and Skoog(M&S) Basal Salt Mixture, only. The M5519 products is M&S Basal Medium which also includes the vitamins myo-Inositol, Nicotinic acid and Pyridoxine HCl. These formulations are identical with the exception of vitamin components. With the addition of 1 mL of M7150 M&S Vitamin Solution, the composition of each medium is equivalent.

      Helpful?

  3. What is the formulation of Product M5519, Murashige and Skoog (MS) Basal Medium?

    1 answer

    1. Please see the link below to review the product datasheet, including the full formulation:

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf

      To review additional Murashige and Skoog formulations, please see the link below:

      https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/plant-tissue-culture/murashige-skoog

      Helpful?

  4. Product M5519, Murashige and Skoog Basal Medium, is on back order. Can you suggest an alternate?

    1 answer

    1. Product M5524 Murashige and Skoog(M&S) Basal Salt Mixture, is an ideal alternative to M5519, M&S Basal Medium. These formulations are identical with the exception of vitamin components. With the addition of 1 mL of M7150 M&S Vitamin Solution, the composition of each medium is equivalent.

      Helpful?

  5. Can I autoclave Product M5519, Murashige and Skoog Basal Medium?

    1 answer

    1. Yes, this medium is autoclavable.

      Helpful?

  6. once the media is prepared, what conditions should it be stored in, and how long can it be stored?

    1 answer

    1. The prepared solution is quite stable but should be stored refrigerated. Monitor the salt solution for particulates or turbidity. After the addition of agar and subsequent sterilization, poured plates or vessels should also be stored aseptically in the refrigerator until use. The shelf life of the stored plates or vessels is most often limited by less stable compounds added to the medium. The end user would have to determine the stability of these components. While there is no firm shelf life on the prepared medium, contamination, and drying are the most common reasons for degradation. Monitor plates/vessels for contaminants or turbidity. An indication of excessive drying will be the gelled medium pulling away from the sides of the plate or vessel. The medium has lost moisture and may not be suitable for use. Moisture loss can be minimized by storing prepared plates/vessels in a sealed container or sealed bags. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf

      Helpful?

  7. What is the percentage of sucrose in M5519?

    1 answer

    1. The M5519 does not contain sucrose. This component would have to be added prior to autoclaving. The typical amount is 30 g/L. See the link below to review the product information sheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf

      Should sucrose be desired, see product M9274, which is more of a complete formulation containing M&S salts, vitamins, sucrose, and agar. See the link below to review the formulations of the various Murashige & Skoog media:
      https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/plant-tissue-culture/murashige-skoog#Basal%20Salts%20Mixtures

      Helpful?

  8. What is the quantity and what is shelf life

    1 answer

    1. The SKU sizes indicated refer to the final volume of media that can be made upon reconstitution of the pre-weighted powder. This powdered media is formulated to contain 4.4 grams of powder per liter of medium. This product does not contain an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services

      Helpful?

  9. How much agar is there in Product M5519, Murashige and Skoog Basal Medium?

    1 answer

    1. This product does not contain any agar.

      Helpful?

  10. Product M5519, Murashige and Skoog Basal Medium, has precipitates; will that affect the growth?

    1 answer

    1. Precipitates are known to occur, with time, in plant tissue culture media.  The precipitates have been analyzed.  They are composed of small pale yellow-white particles.  Analysis of precipitates indicated a predominance of iron, phosphate, and zinc.  The probable cause of the precipitates is the inevitable oxidation of ferrous ions to ferric ions and the presence of unchelated ferric ions.  When the solubility of ferric phosphate is exceeded precipitation occurs.  There are no reports of detrimental effects on growth and development in plant tissue culture due to the precipitates.

      Helpful?

1–10 of 11 Questions  

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