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A5503

Albumin from chicken egg white

lyophilized powder, ≥98% (agarose gel electrophoresis)

Synonym(s):

Albumin from hen egg white, Ovalbumin

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About This Item

CAS Number:
UNSPSC Code:
12352202
eCl@ss:
42010201
EC Number:
232-692-7
NACRES:
NA.25
MDL number:
Biological source:
chicken egg white
Assay:
≥98% (agarose gel electrophoresis)
Form:
lyophilized powder
Technique(s):
bioconjugation: suitable
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biological source

chicken egg white

Quality Segment

type

Grade V

assay

≥98% (agarose gel electrophoresis)

form

lyophilized powder

mol wt

44287 Da by calculation

purified by

crystallization

technique(s)

bioconjugation: suitable

solubility

H2O: soluble 40 mg/mL

UniProt accession no.

storage temp.

2-8°C

Gene Information

chicken ... OVAL(396058)

General description

Chicken egg albumin, or ovalbumin, is the major protein constituent of egg whites. Chicken egg albumin is a phosphorylated glycoprotein. From the amino acid sequence, the peptide portion of ovalbumin consists of 385 residues and has a molecular weight of 42.7 kDa.[1]

Application

A phosphorylated glycoprotein which is useful as a marker (~45 kDa) for protein electrophoresis. Has been used to produce an animal model of asthma.
Ovalbumin is useful as a marker (∼45 kDa) for protein electrophoresis. Ovalbumin has been used to produce an animal model of asthma. Ovalbumin was used as a medium for cell incubation in EliCell assay for the detection of released cytokines from eosinophils.[2]
Studies to test the effects of localization on DNA immunizations use ovalbumin in antibody assays.[3]
Ovalbumin has been used as an induction agent in model studies of atopic dermatitis.

Biochem/physiol Actions

Differential scanning calorimetry indicates that chicken egg albumin denatures at 84 °C.[4] Ovalbumin can be used as a carrier protein to conjugate to synthetic peptides for use as an immunogen.

Physical form

Essentially salt free

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This Item
A2512A7642A5378
biological source

chicken egg white

biological source

chicken egg white

biological source

chicken egg white

biological source

chicken egg white

assay

≥98% (agarose gel electrophoresis)

assay

≥98% (agarose gel electrophoresis)

assay

-

assay

≥90% (agarose gel electrophoresis)

technique(s)

bioconjugation: suitable

technique(s)

bioconjugation: suitable

technique(s)

electrophoresis: suitable

technique(s)

bioconjugation: suitable

Quality Level

300

Quality Level

300

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

powder

form

lyophilized powder

Gene Information

chicken ... OVAL(396058)

Gene Information

chicken ... OVAL(396058)

Gene Information

chicken ... OVAL(396058)

Gene Information

chicken ... OVAL(396058)


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pictograms

Health hazard

signalword

Danger

hcodes

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Hazard Classifications

Resp. Sens. 1



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Questions

1–10 of 10 Questions  
  1. Upon resuspension, at what temperature should albumin be stored and how long is it stable?

    1 answer
    1. Solutions may be stored refrigerated at 2-8°C. The long term stability of this product in solution has not been determined. However, filter sterilized solutions of human and bovine origin have a shelf life of 2 - 3 years when stored refrigerated. Albumin solutions may also be stored at -20 °C. Avoid repeated freeze/thaw cycles for frozen solutions.

      Helpful?

  2. Is this endotoxin free ovalbumin?

    1 answer
    1. Unfortunately, this product is not tested for endotoxin.

      Helpful?

  3. Is A5503 fluorescent?

    1 answer
    1. This product is pure ovalbumin with a minimum purity specification of 98%. No fluorescent conjugate or probe is present in this material.

      Helpful?

  4. Does A5503 can analyze by western blotting?

    1 answer
    1. This product has not been specifically tested for use in western blot. However, there is no reason to suspect that this albumin would not be suitable.

      Helpful?

  5. Is Product No. A5503, Albumin from chicken egg white, suitable for cell culture or injection?

    1 answer
    1. We do not test any of the ovalbumins in cell culture or in vivo applications. Customers would need to perform suitability testing in their own experiments.

      Helpful?

  6. What is the solubility of Product No. A5503, Albumin from chicken egg white, in aqueous buffers (e.g. Tris, PBS)?

    1 answer
    1. We have only tested solubility in water. Salts in buffers may make solubility more difficult.

      Helpful?

  7. Product No. A5503, Albumin from chicken egg white, is to be stored at 2 - 8°C. Does it ship on ice?

    1 answer
    1. A5503 does not require shipping on ice.

      Helpful?

  8. Is Product No. A5503, Albumin from chicken egg white, endotoxin-tested?

    1 answer
    1. A5503 is not endotoxin-tested. None of the ovalbumins are endotoxin-tested.

      Helpful?

  9. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  10. How is Product No. A5503, Albumin from chicken egg white, analyzed for purity?

    1 answer
    1. A5503 is analyzed by agarose gel electrophoresis. We have not done SDS-PAGE.

      Helpful?

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