07-108

Anti-Histone H4 Antibody

Upstate^®, from rabbit

• Synonym(s): H4 histone family, member A, histone 1, H4a, histone cluster 1, H4a
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 161

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: Xenopus, mouse, avian, chicken, human, bovine
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_778224
• UniProt accession no.: P62805
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: bovine ... H4C1(617905) ; frog ... H4C1(549623) ; human ... H4C1(8359) ; mouse ... H4C1(326619)

Description

General description

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

10 kDa

Immunogen

Calf thymus histone H4.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-Histone H4 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect Histone H4 also known as Histone H4.

Biochem/physiol Actions

Broad species cross-reactivity expected.

Recognizes Histone H4, Mr 10 kDa.

Physical form

Format: Purified

Protein A chromatography

Purified rabbit polyclonal IgG in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% Sodium Azide.

Preparation Note

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Analysis Note

Control
HeLa cell extract.

Routinely evaluated by western blot on acid extracts of HeLa cells treated with sodium butyrate.

Western Blot Analysis:
0.1-0.5 μg/mL of this lot detected Histone H4 in acid extracts of HeLa cells treated with or without 5 mM sodium butyrate. This lot did not cross react with Histone H2A, Histone H2B or Histone H3.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable