D8783

7-Deaza-2′-deoxyguanosine 5′-triphosphate lithium salt

10 mM in H₂O

• Synonym(s): −N⁷-dGTP, 7-Deaza-dGTP
• Empirical Formula (Hill Notation): C₁₁H₁₇N₄O₁₃P₃
• CAS Number: 101515-08-6
• Molecular Weight: 506.19
• NACRES: NA.52
• PubChem Substance ID: 24894227
• UNSPSC Code: 41106305
• MDL number: MFCD00171614
• Assay: ≥95% (HPLC)
• Biological source: Porcine muscle, rabbit muscle
• Form: liquid
• Storage temp.: −20°C

Properties

• Quality Segment: 200
• biological source: Porcine muscle, rabbit muscle
• assay: ≥95% (HPLC)
• form: liquid
• feature: PCR Additives
• concentration: 10 mM in H₂O
• technique(s): PCR: suitable
• color: colorless
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: NC1=NC(=O)c2ccn(C3CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O3)c2N1
• InChI: 1S/C11H17N4O13P3/c12-11-13-9-5(10(17)14-11)1-2-15(9)8-3-6(16)7(26-8)4-25-30(21,22)28-31(23,24)27-29(18,19)20/h1-2,6-8,16H,3-4H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,12,13,14,17)
• InChI key: DLLXAZJTLIUPAI-UHFFFAOYSA-N

Description

Application

7-Deaza-2′-deoxyguanosine 5′-triphosphate lithium salt has been used as a lysis agent to evaluate its efficiency on direct cell lysis, RNA stability, and compatibility with downstream reverse transcription quantitative real-time PCR during the analyzes of samples with small numbers of cells.[1]

Biochem/physiol Actions

7-Deaza-2′-deoxyguanosine 5′-triphosphate (7-deaza-dGTP) competes with natural substrate dGTP and blocks the telomerase activity. This nucleotide once incorporated into the telomere alters the secondary structure of the telomere making it difficult for telomerase to identify it for further telomere synthesis.[2] This dGTP analog pairs weakly with conventional bases to minimize DNA secondary structures, while being a good substrate for DNA polymerases. 7-deaza-dGTP in combination with dimethyl sulfoxide (DMSO) and betaine enhances the polymerase chain reaction (PCR) amplification of GC-rich DNA sequences.[3]

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable