This item is not Sterile. Sterile products are typically labeled as "sterile” or “sterile-filtered". Currently, a sterile Chondroitinase product is not offered.
C3667
Chondroitinase ABC from Proteus vulgaris
BSA free, lyophilized powder, specific activity 50-250 units/mg protein
Synonym(s):
4.2.2.20, 4.2.2.21, Chondroitin ABC Lyase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-777-9
MDL number:
Specific activity:
50-250 units/mg protein
Biological source:
bacterial (Proteus vulgaris)
Pricing and availability is not currently available.
biological source
bacterial (Proteus vulgaris)
Quality Level
conjugate
(Glucosaminoglycan)
form
lyophilized powder
specific activity
50-250 units/mg protein
mol wt
120 kDa by gel filtration
composition
Protein, ~10% Lowry
solubility
0.01% bovine serum albumin aqueous (BSA) solution: soluble
application(s)
diagnostic assay manufacturing
foreign activity
protease, essentially free
storage temp.
−20°C
Application
The enzyme has been used to study the consequences of inducing acute, long-lasting changes in chondroitin sulfate proteoglycans using adult rat brain cells.[1] Glycosaminoglycans have been digested with chondroitinase ABC for chondroitin/dermatan sulfate quantitation in keratocyte cell cultures obtained from fresh bovine corneal stromae.[2]
Biochem/physiol Actions
Chondroitinase ABC catalyzes the eliminative degradation of polysaccharides containing (1-4)-β-D-hexosaminyl and (1-3)-β-D-glucuronosyl or (1-3)-α-L-iduronosyl linkages to disaccharides containing 4-deoxy-β-D-gluc-4-enuronosyl groups. It acts on chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate, and acts slowly on hyaluronate. The molecular weight is found to be approximately 120 kDa with 2 non-identical subunits of molecular masses 86 kDa and 32 kDa. The pH optimum is found to be 8.0 with chondroitin sulfate and 6.8 with hyaluronic acid and temperature optimum is 37 °C. 1 mM Zn2+ acts as an inhibitor, while 0.05 M acetate is an activator of the enzyme.
Packaging
Packages based on chondroitinase C activity.
Preparation Note
Affinity purified
Other Notes
Contains potassium phosphate buffer salts and stabilizer
One unit will liberate 1.0 μmole of a mixture of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-4-O-sulfo-D-galactose and 1.0 μmole of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-6-O-sulfo-D-galactose from chondroitin sulfate from shark cartilage, per min at pH 8.0 at 37 °C.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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This Item | |||
|---|---|---|---|
| specific activity 50-250 units/mg protein | specific activity 0.3-3 units/mg solid | specific activity 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate) | specific activity 50-250 units/mg protein |
| biological source bacterial (Proteus vulgaris) | biological source bacterial (Proteus vulgaris) | biological source - | biological source bacterial (Proteus vulgaris) |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form - |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) - |
| mol wt 120 kDa by gel filtration | mol wt ~120 kDa by gel filtration | mol wt - | mol wt - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −70°C |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
P N Bansal et al.
Osteoarthritis and cartilage, 18(2), 184-191 (2009-10-10)
An early hallmark of osteoarthritis (OA) is the progressive loss of glycosaminoglycans (GAGs), the extracellular matrix (ECM) component of articular cartilage that confers it with compressive stiffness. Our aim in this work is to establish the feasibility of using Contrast
Susan M Smith et al.
International journal of molecular sciences, 20(3) (2019-01-27)
The aim of this study was to assess if the ovine articular cartilage serine proteinase inhibitors (SPIs) were related to the Kunitz inter-α-trypsin inhibitor (ITI) family. Ovine articular cartilage was finely diced and extracted in 6 M urea and SPIs
Elizabeth J Bradbury et al.
Brain research bulletin, 84(4-5), 306-316 (2010-07-14)
Chondroitin sulphate proteoglycans (CSPGs) are potent inhibitors of growth in the adult CNS. Use of the enzyme chondroitinase ABC (ChABC) as a strategy to reduce CSPG inhibition in experimental models of spinal cord injury has led to observations of a
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C3667-5UN | 04061833486221 |
| C3667-10UN | 04061833486214 |
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Is this product aseptic/sterilized？
1 answer-
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Could you provide specific data regarding the pH-dependent activity of Chondroitinase ABC (Product No. C3637)? I am particularly interested in how the enzymatic activity changes between pH 7.0 and pH 8.0.
1 answer-
This product shows maximal enzymatic activity on chondroitin sulfate at approximately pH 8.0, based on assay conditions at 37 °C. At pH 7.0, the enzymatic activity rate is significantly lower. The pH-activity profile indicates activity peaks at pH 8.0 and decreases to less than 40% when the pH falls below 7.0, indicating a steep decline. For hyaluronan, the optimal pH is approximately 6.8. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/399/066/c3667pis-ms.pdfPlease see the following link to review a publication demonstrating the impact of pH on the activity of Chondroitinase ABC
https://www.sciencedirect.com/science/article/pii/S0008621510004702Helpful?
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Good evening, in which solution has to be solubilized the product? Thanks a lot, Sara
1 answer-
This product should be reconstituted in a 0.01% BSA aqueous solution. Subsequent dilutions can be made into a buffer containing 50 mM Trizma® HCl, pH 8.0, with 60 mM sodium acetate and 0.02% BSA Prepare solutions just prior to use. More product details are available under "More Documents" or at this link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/399/066/c3667pis-ms.pdf
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Could you clarify which enzymes are in the product C3667?
1 answer-
The product containing Chondroitinase ABC purified from Proteus vulgaris is a mixture of two enzymes: chondroitin-sulfate-ABC endolyase and chondroitin-sulfate-ABC exolyase.
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