This product is soluble in water at a concentration of 2 mg/ml, resulting in a clear solution. Concanavalin A from Canavalia ensiformis (Jack bean) has been utilized in the colabeling of Fischer rat thyroid epithelial cells, to induce autoimmune hepatitis in Cre-conditional atg7 knockout mice, and to detect glycoproteins containing α-D-mannose and β-D-glucose when used with an avidin or streptavidin detection system. This product has not been tested or validated for immunoprecipitation experiments. It is recommended to consult literature for procedures to isolate plasma membrane surface proteins using Concanavalin A.
C2272
Concanavalin A from Canavalia ensiformis (Jack bean)
biotin conjugate, Type IV, lyophilized powder
Concanavalin A from Canavalia ensiformis (Jack bean)
Synonym(s):
ConA
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
Biological source:
Canavalia ensiformis
Pricing and availability is not currently available.
biological source
Canavalia ensiformis
Quality Level
conjugate
biotin conjugate
type
Type IV
form
lyophilized powder
composition
Protein, ~85% ε1%/280
extent of labeling
4-8 mol biotin per mol protein
impurities
Carbohydrate, essentially free
storage temp.
2-8°C
General description
Concanavalin A is isolated from the from Canavalia ensiformis (Jack bean). It is a mitogenic lectin with a dome-like structures and is a tetrameric protein.
Application
Concanavalin A from Canavalia ensiformis (Jack bean) has been used:
- in the colabelling of fischer rat thyroid (FRT) epithelia(104)
- to induce autoimmune hepatitis in Cre-conditional atg7 knockout mice(105)
- to detect glycoproteins containing α-D-mannose, α-D-glucose when used with avidin or streptavidin detection system(106)
Biochem/physiol Actions
Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Concanavalin A (Con A) bound to glycoenzyme has wide applications and can be dissociated in the presence of high sugar levels. Con A induces hepatic inflammation. It stimulates macrophages and promotes cytokine production. Con A also exhibits anti-hepatoma effect and could be used effectively as a vaccine for treating cancer.
Packaging
Package size based on protein content
Physical form
Contains sodium citrate
Analysis Note
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
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This Item | |||
|---|---|---|---|
| biological source Canavalia ensiformis | biological source - | biological source Canavalia ensiformis | biological source - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| impurities Carbohydrate, essentially free | impurities Carbohydrate, essentially free, salt, essentially free | impurities Carbohydrate, essentially free | impurities salt (Contains buffer salts and NaCl) |
| conjugate biotin conjugate | conjugate - | conjugate FITC conjugate | conjugate - |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
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Hillary Claire Tunggal et al.
PloS one, 16(6), e0253265-e0253265 (2021-06-18)
A therapeutic vaccine that induces lasting control of HIV infection could eliminate the need for lifelong adherence to antiretroviral therapy. This study investigated a therapeutic DNA vaccine delivered with a single adjuvant or a novel combination of adjuvants to augment
Structure and function of concanavalin A
Reeke GN, et al.
Advances in Experimental Medicine and Biology, 55, 13-33 (1975)
Atg7 knockdown augments concanavalin A-induced acute hepatitis through an ROS-mediated p38/MAPK pathway
Zhuang Y, et al.
PLoS ONE, 11(3), e0149754-e0149754 (2016)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C2272-10MG | 04061833475539 |
| C2272-2MG | 04061833475546 |
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What agent should be used to resolve conA powder? Is this type of conA suitable for isolating the proteins on the surface of the plasma membrane? What concentration of conA should be used in IP experiments for isolating plasma membrane surface proteins?
1 answer-
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What solvent does this product dissolve in?
1 answer-
This product is soluble in water up to 10 mg/mL and may yield a slightly hazy, colorless to faint yellow solution. The solubility may be increased by the addition of sodium chloride.
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