B7166

5-Bromouridine 5′-triphosphate sodium salt

≥93%, synthetic (organic), powder

• Synonym(s): 5-BrUTP
• Empirical Formula (Hill Notation): C₉H₁₄BrN₂O₁₅P₃ · xNa⁺
• CAS Number: 161848-60-8
• Molecular Weight: 563.04 (free acid basis)
• UNSPSC Code: 41106305
• PubChem Substance ID: 24891977
• NACRES: NA.51
• Assay: ≥93%
• Biological source: synthetic (organic)
• Form: powder
• Solubility: water: 50 mg/mL, clear, colorless
• Storage temp.: −20°C

Properties

• Product Name: 5-Bromouridine 5′-triphosphate sodium salt, ≥93%
• biological source: synthetic (organic)
• Quality Level: 200
• assay: ≥93%
• form: powder
• solubility: water: 50 mg/mL, clear, colorless
• storage temp.: −20°C
• SMILES string: OP(OP(OP(O)(O)=O)(O)=O)(OC[C@H]1O[C@@H](N(C=C2Br)C(NC2=O)=O)[C@H](O)[C@@H]1O)=O.[Na]
• InChI: 1S/3Na/q3*+1
• InChI key: BVPYARCRLTXGBD-UHFFFAOYSA-N

Description

General description

5-Bromouridine 5′-triphosphate (5-BrUTP) is used to measure transcription via labeling of ribonucleic acids (RNA). RNA or cells labeled via 5-BrUTP incorporation may be detected immunologically with antibodies.[1]

Application

5-Bromouridine 5′-triphosphate (5-BrUTP) is used to measure transcription via labeling of ribonucleic acids (RNA). RNA or cells labeled via 5-BrUTP incorporation may be detected immunologically with antibodies.

5-Bromouridine 5′-triphosphate sodium salt has been used:

• to infer the chromosome intermingling between embryonic stem (ES) cells (differentiated and undifferentiated) and terminally differentiated NIH3T3 cells[2]
• to label nascent RNA in in vivo transcription analysis using fibroblasts[3]
• and transfected MDA-MB-231 cells[4]
• to label RNA for nuclear run-on experiments[5]

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• ppe: dust mask type N95 (US), Eyeshields, Gloves