This product may be stored at room temperature.
83702
Rhodamine 123
BioReagent, suitable for fluorescence, ≥85% (HPLC)
Synonym(s):
2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester
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About This Item
Linear Formula:
C21H17ClN2O3
CAS Number:
Molecular Weight:
380.82
UNSPSC Code:
12352116
NACRES:
NA.32
PubChem Substance ID:
EC Number:
263-687-8
Beilstein/REAXYS Number:
6030951
MDL number:
Pricing and availability is not currently available.
product line
BioReagent
Quality Level
assay
≥85% (HPLC)
form
powder
impurities
≤10% water
solubility
ethanol: 20 mg/mL, clear, red
suitability
in accordance for fluorescence, suitable for fluorescence
SMILES string
Cl[H].COC(=O)c1ccccc1C2=C3C=CC(=N)C=C3Oc4cc(N)ccc24
InChI
1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H
InChI key
MYFATKRONKHHQL-UHFFFAOYSA-N
General description
Rhodamine 123 is a fluorescent dye.
Application
Rhodamine 123 has been used:
- as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assays
- to label the cells for estimating the P-gp expression by flow cytometry
- in transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport)
- the detection of mitochondrial membrane potential (ΔΨm)
Biochem/physiol Actions
Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.
Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry.
Other Notes
Supravital mitochondrial-specific dye[1]
1 of 1
This Item | |||
|---|---|---|---|
| suitability in accordance for fluorescence, suitable for fluorescence | suitability in accordance for fluorescence, suitable for fluorescence | suitability - | suitability - |
| form powder | form powder or crystals | form powder | form crystals |
| solubility ethanol: 20 mg/mL, clear, red | solubility H2O: soluble, methanol: soluble | solubility ethanol: 1 mg/mL | solubility ethanol: soluble, methanol: soluble |
| assay ≥85% (HPLC) | assay - | assay - | assay ≥99.0% (UV) |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| product line BioReagent | product line - | product line - | product line BioReagent |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Assessment of the chemotherapeutic potential of a new camptothecin derivative, ZBH-1205
Wu D, et al.
Archives of Biochemistry and Biophysics, 604, 74-85 (2016)
Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells
Stebbins MJ, et al.
Methods, 101, 93-102 (2016)
X Ronot et al.
Biology of the cell, 57(1), 1-7 (1986-01-01)
Flow cytometry combines the advantages of microscopy and biochemical analysis in a single highly sensitive technique for a rapid examination of numerous individual living cells. It has become a potent and essential tool in the studies of the physiology of
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 83702-10MG | 04061833005781 |
| 83702-50MG | 04061833005798 |
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Which is the storage conditions recommend for this product?
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Why would one select Rhodamine 123 (Product Nos. 83702 and R8004) instead of other rhodamine compounds?
1 answer-
Due to is comparatively low toxicity, Rhodamine 123 is the dye of choice for visualizing mitochondria or for cell viability measurements.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the solubility of Rhodamine 123, (Product Nos. 83702 and R8004)?
1 answer-
Rhodamine 123 is soluble in ethanol at 20 mg/mL, and has been reported soluble in ether, various fats and lipids, and has limited solubility in water (no concentration given).
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What are the excitation and emission wavelengths for Rhodamine 123 (Product Nos. 83702 and R8004)?
1 answer-
Using ethanol as the solvent, the excitation maximum is 511 nm and the emission maximum is 534 nm. The recommended excitation and emission wavelengths in PBS are 488 and 515-575 nm, respectively.
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