A5170

Anti-Avidin antibody produced in rabbit

whole antiserum

• UNSPSC Code: 12352203
• NACRES: NA.43
• MDL number: MFCD00162144
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ELISA (i), IEP
• Citations: 7

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: whole antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• contains: 15 mM sodium azide
• technique(s): immunoelectrophoresis: suitable, indirect ELISA: 1:100,000
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties. Avidin from corn has low molecular weight than chicken egg-derived avidin. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.

Immunogen

avidin

Application

Anti-Avidin antibody produced in rabbit has been used in enzyme-linked immunosorbent assay (ELISA). It has also been used in preparation of antibody-Au nanoparticles.

The presence of Scavidin in BT4C glioma cells was assessed by western blot using a rabbit anti-avidin antibody as the primary at a dilution of 1:4000. Lehtolainen, P. (2002) Cloning and Characterization of Scavidin, a Fusion Protein for the Targeted Delivery of Biotinylated Molecules. J. Biol. Chem. 277:8545-8550.

Biochem/physiol Actions

Avidin binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Physical form

Supplied as a liquid containing 15mM sodium azide as preservative

Preparation Note

treated to remove lipoproteins

Analysis Note

This antisera is evaluated for performance and specificity by immunodiffusion and immunoelectrophoresis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable