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A3660

Apoferritin from equine spleen

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About This Item

CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.25
MDL number:
Form:
glycerol solution (50% glycerol and 0.075 M NaCl)
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form

glycerol solution (50% glycerol and 0.075 M NaCl)

Quality Level

packaging

vial of 50 mg

storage temp.

−20°C

Application

Apoferritin is a gel filtration molecular weight marker that can be used in gel filtration chromatography and protein chromatography. Apoferritin has the ability to demetallate hemin and to metallate protoporphyrin IX.

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This Item
A3641F4503L1668
form

glycerol solution (50% glycerol and 0.075 M NaCl)

form

liquid

form

saline solution

form

lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

packaging

vial of 50 mg

packaging

-

packaging

-

packaging

-


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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Noëlle Carette et al.
Journal of inorganic biochemistry, 100(8), 1426-1435 (2006-06-20)
Earlier crystallographic and spectroscopic studies had shown that horse spleen apoferritin was capable of removing the metal ion from hemin (Fe(III)-protoporphyrin IX) [G. Précigoux, J. Yariv, B. Gallois, A. Dautant, C. Courseille, B. Langlois d'Estaintot, Acta Cryst. D50 (1994) 739-743;
David P Klebl et al.
Acta crystallographica. Section D, Structural biology, 76(Pt 4), 340-349 (2020-04-08)
Despite the great strides made in the field of single-particle cryogenic electron microscopy (cryo-EM) in microscope design, direct electron detectors and new processing suites, the area of sample preparation is still far from ideal. Traditionally, sample preparation involves blotting, which
Venkata P Dandey et al.
Journal of structural biology, 202(2), 161-169 (2018-01-26)
We present an update describing new features and applications of Spotiton, a novel instrument for vitrifying samples for cryoEM. We have used Spotiton to prepare several test specimens that can be reconstructed using routine single particle analysis to ∼3 Å resolution



Global Trade Item Number

SKUGTIN
A3660-5X1VL04061838219541
A3660-1VL04061833367759

Questions

1–6 of 6 Questions  
  1. What is the density of A3660-1vl (Apoferritin from equine spleen)?

    1 answer
    1. The solution is 50% glycerol with 75mM NaCL, in addition to the presence of 50 mg of Apoferritin per vial. However, a 50% glycerol solution is typically expected to have a density of 1.26 g/ml.

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  2. What is the purity percentage of A3660?

    1 answer
    1. Unfortunately, this product is not tested for purity.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. Can Product A3660, Apoferritin from equine spleen, be sterilized?

    1 answer
    1. Yes. First the glycerol will need to be removed (typically by dialysis). Then a solution of apoferritin in 0.15M sodium chloride can be filtered through a 0.2 micron filter, as is done for Product No. A3641.

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  5. What is the molecular weight of Product A3660, Apoferritin from equine spleen?

    1 answer
    1. The approximate molecular weight of this product is 443,000.

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  6. What is the concentration of Product A3660, Apoferritin from equine spleen?

    1 answer
    1. The concentration is roughly 25 mg/mL, according to the technical bulletin.

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