TRIS borate – EDTA buffer solution

Name: TRIS borate – EDTA buffer solution
Brand: SIGMA

for Northern and Southern blotting, solution

Synonym(s):

TBE buffer

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About This Item

UNSPSC Code:

41105319

NACRES:

NA.25

eCl@ss:

32129211

Pricing and availability is not currently available.

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Properties

grade

Molecular Biology

Quality Level

100

description

long run, concentrate

product line

BioReagent

form

solution

quality

10× concentrate, ready-to-use solution

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

pH

8.6-9.0

storage temp.

room temp

Description

General description

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. The TBE solution is effective under slightly basic conditions, which keeps DNA deprotonated , water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).

Application

Suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures.

Preparation Note

The TBE 10x liquid contains 1.3M TRIS, 450 mM boric acid and 25 mM EDTA in water.

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This Item	51309	T4415	T9285
Sigma-Aldrich 93290 TRIS borate – EDTA buffer solution	Sigma-Aldrich 51309 TRIS borate-EDTA buffer concentrate, long run	Sigma-Aldrich T4415 Tris-Borate-EDTA buffer	Sigma-Aldrich T9285 Tris-EDTA buffer solution
grade Molecular Biology	grade Molecular Biology	grade -	grade Molecular Biology
product line BioReagent	product line BioReagent	product line BioReagent	product line -
Quality Level 100	Quality Level 100	Quality Level 200	Quality Level 200
form solution	form solution	form solution	form liquid
pH 8.6-9.0	pH -	pH -	pH 7.8-8.2 (1 × in water)
storage temp. room temp	storage temp. room temp	storage temp. -	storage temp. room temp