TMB is a chromogenic substrate for horseradish peroxidase (HRP). The complete formulation of this product is considered proprietary. However, the color change to blue/purple is due to the oxidation of the TMB substrate, which is catalyzed by peroxidase.
T9455
TMB Enhanced One Component HRP Membrane Substrate
Synonym(s):
HRP substrate, TMB, detection reagent, detection substrate
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About This Item
NACRES:
NA.83
UNSPSC Code:
12352204
Pricing and availability is not currently available.
General description
TMB Enhanced One Component HRP Membrane Substrate is a ready-to-use solution containing 3,3′,5,5′-tetramethylbenzidine (TMB) in a mildly acidic buffer. TMB is a substrate for horseradish peroxidase. The substrate system will react with sites on the membrane containing peroxidase, producing an insoluble permanent dark blue reaction product. To stop the reaction, rinse the membrane with reagent quality water. This substrate is not recommended for microwell or immunohistochemical applications.
Application
TMB Enhanced One Component HRP Membrane Substrate has been used as a colorimetric substrate for horseradish peroxidase (HRP) in an electrochemical enzymatic sandwich assay for the detection of interleukin-6 (IL-6) in biological fluids.[1]
Physical form
Ready-to-use.
Disclaimer
Stable at least one year at 2-8 °C.
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This Item | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 100 | Quality Level - | Quality Level 100 |
| form solution | form liquid | form liquid | form liquid |
| storage temp. 2-8°C | storage temp. - | storage temp. - | storage temp. 2-8°C |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| T9455-1L | 04061837381447 |
| T9455-100ML | 04061837381416 |
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The webpage states it produces a deep blue/purple product with HRP, and the unreacted substrate should be pale yellow to pink. Our professor wants to know the components causing this purple color. Could you please look into this for us? Thank you.
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