This product is a reagent that combines soluble TMB chromogen, buffer, and hydrogen peroxide in a convenient, ready-to-use, single solution system. No additional dilution or ingredients are required.
T8665
3,3′,5,5′-Tetramethylbenzidine
peroxidase substrate, chromogenic, liquid
Synonym(s):
TMB
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.83
Pricing and availability is not currently available.
Product Name
3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System, peroxidase substrate
Quality Segment
form
liquid
storage condition
protect from light
shipped in
wet ice
storage temp.
2-8°C
Application
TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.
3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.
Physical form
Ready-to-use.
Disclaimer
This substrate is very light-sensitive and should be protected from direct sunlight or UV sources.
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This Item | |||
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| Quality Level 300 | Quality Level 300 | Quality Level - | Quality Level 200 |
| form liquid | form liquid | form liquid | form aqueous solution |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| storage condition protect from light | storage condition - | storage condition - | storage condition - |
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Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 3 - STOT SE 1
target_organs
Eyes,Central nervous system
wgk
WGK 2
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Do we need to add hyrodgen peroxide when reacting with HRP?
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What is the concentration of 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate?
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The concentration of this item is 1.6mM.
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