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Trypsin from human pancreas

Name: Trypsin from human pancreas
Brand: SIGMA

salt-free, lyophilized powder, vial of ≥1,000 BAEE units

Synonym(s):

Serine Protease 1

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About This Item

CAS Number:

9002-07-7

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-650-8

MDL number:

MFCD01323069

EC Number:

3.4.21.4 (BRENDA, IUBMB)

Biological source:

human pancreas

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Properties

biological source

human pancreas

Quality Segment

200

form

salt-free, lyophilized powder

packaging

vial of ≥1,000 BAEE units

UniProt accession no.

P07477, P07478, P35030

storage temp.

−20°C

Gene Information

human ... PRSS1(5644), PRSS2(5645), PRSS3(5646)

Description

General description

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Trypsin is a serine protease. The pancreas produce the precursor trypsinogen and secretes it to the intestine. Trypsinogen is converted to trypsin by enterokinase in the intestinal mucous membrane.

Application

Trypsin has been used in a study to assess the similarities between the hepatitis E virus and human astrovirus. [1] Trypsin has also been used in a study to characterize a unique technique for culturing primary adult human epithelial progenitor, or stem, cells. [2]

Preparation Note

Minimum 1,000 BAEE units/vial

Analysis Note

Protein determined by E1%/280.

Other Notes

One BAEE unit will produce a ΔA₂₅₃ of 0.001 per min at pH 7.6 at 25 °C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer

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This Item	T4799	T7168	T7409
Sigma-Aldrich T6424 Trypsin from human pancreas	Sigma-Aldrich T4799 Trypsin from porcine pancreas	Sigma-Aldrich T7168 Trypsin from porcine pancreas	Sigma-Aldrich T7409 Trypsin from porcine pancreas
biological source human pancreas	biological source Porcine pancreas	biological source Porcine pancreas	biological source -
Gene Information human ... PRSS1(5644), PRSS2(5645), PRSS3(5646)	Gene Information -	Gene Information -	Gene Information -
form salt-free, lyophilized powder	form lyophilized powder	form tablet	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
UniProt accession no. P07477, P35030, P07478	UniProt accession no. -	UniProt accession no. -	UniProt accession no. -
packaging vial of ≥1,000 BAEE units	packaging -	packaging -	packaging -