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Triosephosphate Isomerase from rabbit muscle

Name: Triosephosphate Isomerase from rabbit muscle
Brand: SIGMA

Type X, lyophilized powder, ≥3,500 units/mg protein

Synonym(s):

D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI

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About This Item

CAS Number:

9023-78-3

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-774-2

MDL number:

MFCD00082080

EC Number:

5.3.1.1 (BRENDA, IUBMB)

Specific activity:

≥3,500 units/mg protein

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Properties

type

Type X

Quality Segment

200

form

lyophilized powder

specific activity

≥3,500 units/mg protein

mol wt

calculated mol wt 53.2 kDa

composition

Protein, 60-90% biuret

foreign activity

pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%

storage temp.

−20°C

Description

General description

Triosephosphate isomerase (TPI) gene is located on human chromosome 12p13. It is expressed in all tissues. This homodimer of two 27 kDa subunits is highly stable and is made up of 248 amino acids. TPI is catalytically active in its dimeric state.

Application

Triosephosphate Isomerase from rabbit muscle has been complexed with p-hydroxymercurybenzoate (PHMB) to denature the protein for hydrophobic interaction chromatography coupled online with chemical vapour atomic fluorescence spectrometry (HIC−CVGAFS).

Triosephosphate isomerase has been used in a study to assess changes in cardiac energy metabolic pathways in overweight rats fed a high-fat diet. Triosephosphate isomerase has also been used in a study to investigate proteomic identification of carbonylated proteins in F344 rat hippocampus after 1-bromopropane exposure. [1]

Biochem/physiol Actions

Triosephosphate isomerase (TPI) is responsible for the catalysis of the triose phosphate isomers, dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. TPI deficiency is associated with glycolytic enzymopathy. Lack of TPI is also associated with chronic hemolytic anemia, enhanced exposure to bacterial infections, cardiomyopathy and neuromuscular disease.

Packaging

Package size based on protein content

Physical form

Sulfate-free, contains EDTA and borate buffer salts

Other Notes

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

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This Item	T2391	P9544	P6635
Sigma-Aldrich T6258 Triosephosphate Isomerase from rabbit muscle	Sigma-Aldrich T2391 Triosephosphate Isomerase from rabbit muscle	Sigma-Aldrich P9544 Phosphoglucose Isomerase from rabbit muscle	Sigma-Aldrich P6635 Phosphorylase b from rabbit muscle
specific activity ≥3,500 units/mg protein	specific activity ≥4,000 units/mg protein	specific activity ≥200 units/mg protein	specific activity ≥20 units/mg protein
form lyophilized powder	form ammonium sulfate suspension	form lyophilized powder	form lyophilized powder
mol wt calculated mol wt 53.2 kDa	mol wt calculated mol wt 53.2 kDa	mol wt -	mol wt 97,200 Da by calculation
storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 300
foreign activity pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%	foreign activity 3-phosphoglyceric phosphokinase ≤0.01%, glyceraldehyde-3-phosphate dehydrogenase ≤0.01%, pyruvate kinase, lactic dehydrogenase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase and aldolase ≤0.001%	foreign activity -	foreign activity phosphoglucomutase ≤1.0%, phosphorylase a ≤10%, phosphorylase kinase ≤0.5%, phosphorylase phosphatase, debrancher enzyme, AMPase and ATPase ≤0.1%

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000541926

0000518467

0000505602

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Global Trade Item Number

SKU	GTIN
T6258-1MG	04061837358517
T6258-5MG	04061833274682

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