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T3073

Tissue Preparation Solution

30 mL sufficient for 1,000 extractions

Tissue Preparation Solution

Synonym(s):

Direct PCR tissue preparation solution, Extract-N-Amp Tissue Preparation Solution, REDExtract-N-Amp Tissue Preparation Solution, SYBR Green Extract-N-Amp Tissue Preparation Solution

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About This Item

UNSPSC Code:
12352200
EC Number:
254-457-8
NACRES:
NA.55
Pricing and availability is not currently available.
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Quality Level

usage

30 mL sufficient for 1,000 extractions, sufficient for 1000 reactions

feature

Direct PCR

technique(s)

PCR: suitable

input

crude DNA

shipped in

wet ice

storage temp.

−20°C

General description

The Tissue Preparation Solution is intended for use with the Extract-N-Amp Tissue PCR kits, REDExtract-N-Amp Tissue PCR kits, and SYBR Green Extract-N-Amp Tissue PCR kits. This product is intended to supplement the kit reagents, where there are many extractions.

Application

Tissue preparation solution has been used:
  • in combining segments of tails for genotyping[1]
  • to extract genomic DNA from zebrafish embryos/fin tissues
  • to extract DNA from ear tags, tail tips and embryonic yolk sacs of mouse embryo
  • for extraction of DNA from fungal spores and mycelia[2]

Legal Information

SYBR is a trademark of Thermo Fisher Scientific or its subsidiaries
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

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This Item
XNATD5688XNAT2
storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

feature

Direct PCR

feature

dNTPs included, hotstart, Direct PCR

feature

Direct PCR

feature

dNTPs included, hotstart, Direct PCR

usage

30 mL sufficient for 1,000 extractions, sufficient for 1000 reactions

usage

sufficient for 10 reactions, 10 reactions sufficient for 10 amplifications, 10 reactions sufficient for 10 extractions, sufficient for 100 reactions, 100 reactions sufficient for 100 amplifications, 100 reactions sufficient for 100 extractions, sufficient for 1000 reactions, 1000 reactions sufficient for 1000 amplifications, 1000 reactions sufficient for 1000 extractions

usage

-

usage

sufficient for 100 amplifications, sufficient for 100 extractions, sufficient for 100 reactions

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

wet ice

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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Titilayo D O Falade et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 33(7), 1241-1253 (2016-06-07)
In vitro experimental environments are used to study interactions between microorganisms, and to predict dynamics in natural ecosystems. This study highlights that experimental in vitro environments should be selected to match closely the natural environment of interest during in vitro
N-cadherin prevents the premature differentiation of anterior heart field progenitors in the pharyngeal mesodermal microenvironment.
Soh BS
Cell Research, 24(12), 1420-1432 (2014)
A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish
Varshney GK, et al.
Nature Protocols, 11(12), 2357-2357 (2016)



Global Trade Item Number

SKUGTIN
T3073-.3ML04061838250131
T3073-3ML04061838101266
T3073-30ML04061833768112

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