STA20

Starch (GO/P) Assay Kit

sufficient for 20 assays

• Synonym(s): Starch Measurement Kit
• NACRES: NA.84
• UNSPSC Code: 12164500

Properties

• usage: sufficient for 20 assays
• Quality Segment: 100
• application(s): food and beverages; general analytical
• storage temp.: 2-8°C

Description

General description

Starch (GO/P) Assay Kit was used to determine the starch content, during an experimental study done in order to investigate the composition and structure of sorghum spent grains (SSG), which is a brewery co-product and is reported to have potential applications thereby adding value to the product. It was also used in starch extraction and quantification, during investigating the importance of OsGA2ox5 in GAs homeostasis, development, gravity responses and stress tolerance in rice. Furthermore, it also found application in being used in analysing factors affecting polyhydroxybutyrate accumulation , which are basically linear biodegradable polyesters produced by bacteria as a carbon store, in mesophyll cells of sugarcane and switchgrass.

Application

Suitable for the quantitative, enzymatic determination of starch infood and other materials.

This kit is for the quantitative, enzymatic determination of starch in food and other materials. The hydrolysis of starch to glucose is catalyzed by α-amylase and amyloglucosidase. Glucose is oxidized to gluconic acid and hydrogen peroxide by glucose oxidase. Hydrogen peroxide reacts with o-dianisidine in the presence of peroxidase to form a colored product. Oxidized o-dianisidine reacts with sulfuric acid to form a more stable colored product. The intensity of the pink color measured at 540 nm is proportional to the original glucose concentration.

Starch (GO/P) Assay Kit was used to determine the starch content, during an experimental study done in order to investigate the composition and structure of sorghum spent grains (SSG), which is a brewery co-product and is reported to have potential applications thereby adding value to the product. It was also used in starch extraction and quantification, during investigating the importance of OsGA2ox5 in GAs homeostasis, development, gravity responses and stress tolerance in rice. Furthermore, it also found application in being used in analysing factors affecting polyhydroxybutyrate accumulation , which are basically linear biodegradable polyesters produced by bacteria as a carbon store, in mesophyll cells of sugarcane and switchgrass.[1]

Biochem/physiol Actions

This kit is for the quantitative, enzymatic determination of starch in food and other materials. The hydrolysis of starch to glucose is catalyzed by α-amylase and amyloglucosidase. Glucose is oxidized to gluconic acid and hydrogen peroxide by glucose oxidase. Hydrogen peroxide reacts with o-dianisidine in the presence of peroxidase to form a colored product. Oxidized o-dianisidine reacts with sulfuric acid to form a more stable colored product. The intensity of the pink color measured at 540 nm is proportional to the original glucose concentration.

Kit Components Only

• α-Amylase, Heat Stable .5 mL
• Starch Assay Reagent 1 mL/vial
• Glucose Oxidase/Peroxidase Reagent 1 capsule
• o-Dianisidine Reagent 5 mg
• Glucose Standard Solution .5 mL
• Wheat Starch 1 g
• Corn Starch 1 g

Safety Information

• pictograms: GHS08,GHS05,GHS07
• signalword: Danger
• hcodes: H302,H314,H334,H335,H350
• pcodes: P202 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• wgk: WGK 3