S8140

SSPE Buffer

for Northern and Southern blotting, powder blend

• Synonym(s): Saline-Sodium Phosphate-EDTA Buffer
• NACRES: NA.25
• UNSPSC Code: 41105319

Properties

• grade: Molecular Biology
• Quality Level: 200
• form: powder blend
• pH: 7.2-7.6 (20 × in water, high purity, ambient)
• solubility: water, high purity: 20 ×, clear, colorless
• foreign activity: DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected
• storage temp.: room temp

Description

General description

SSPE Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. This powder should be reconstituted to the indicated volume with molecular biology grade water before use. The resulting 20x liquid can be used straight or diluted as needed.

Application

• Influence of Saline Buffers over the Stability of High-Annealed Gold Nanoparticles Formed on Coverslips for Biological and Chemosensing Applications.: This study explores the role of saline buffers in maintaining the stability of high-annealed gold nanoparticles used in biological and chemosensing applications. The findings highlight the potential for enhanced sensor performance in detecting biological molecules (Zhou & Ionescu, 2020).


• Quantification of Surface Etching by Common Buffers and Implications on the Accuracy of Label-Free Biological Assays.: This research assesses the impact of various buffers, including SSPE buffer, on the surface etching of substrates used in label-free biological assays. The results indicate that buffer-induced surface changes can significantly affect assay accuracy (Ahn et al., 2012).

Features and Benefits

• Easy to reconstitute powder
• Multi-purpose use in hybridizations

Preparation Note

Final SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges