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S7899

Sodium acetate buffer solution

pH 5.2±0.1 (25 °C), Molecular Biology, 3 M, 0.2 μm filtered

Synonym(s):

Sodium acetate buffer 3M

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About This Item

CAS Number:
NACRES:
NA.52
PubChem Substance ID:
eCl@ss:
32129211
UNSPSC Code:
12161700
MDL number:
Beilstein/REAXYS Number:
3786729
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grade

Molecular Biology

Quality Level

sterility

0.2 μm filtered

form

liquid

concentration

3 M

pH

5.2±0.1 (25 °C)

suitability

suitable for DNA precipitation

storage temp.

room temp

SMILES string

[Na+].CC(O)=O.CC([O-])=O

InChI

1S/2C2H4O2.Na/c2*1-2(3)4;/h2*1H3,(H,3,4);/q;;+1/p-1

InChI key

BHZOKUMUHVTPBX-UHFFFAOYSA-M

General description

Sigma′s Sodium Acetate Buffer is a 3M solution prepared with 18 megaohm water and filter-sterilized.

Application

Sodium acetate buffer solution is suitable for precipitation of DNA, elution of proteins and for use in molecular biology applications such as ChIP-on-ChIP assay[1] and in vitro enzyme inhibition assay.[2][3]

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This Item
5674227119693302
grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

concentration

3 M

concentration

-

concentration

~3 M in H2O

concentration

-

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

100

form

liquid

form

liquid

form

liquid

form

liquid

pH

5.2±0.1 (25 °C)

pH

-

pH

7.0-8.0 (25 °C, 3 M in H2O)

pH

7.4±0.2 (25 °C)

storage temp.

room temp

storage temp.

10-30°C

storage temp.

-

storage temp.

-


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pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Related Content

Product Information Sheet


Tomas Erban et al.
Scientific reports, 7(1), 5084-5084 (2017-07-13)
Honeybee (Apis mellifera L.) workers act as passive vectors of Paenibacillus larvae spores, which cause the quarantine disease American foulbrood (AFB). We assessed the relative proportions of P. larvae within the honeybee microbiome using metabarcoding analysis of the 16 S rRNA
Sambrook, J., and Russell, D.W.
Molecular Cloning: A Laboratory Manual, A1-A1 (2001)
Jaspreet Kaur et al.
Antimicrobial agents and chemotherapy, 55(2), 659-666 (2010-12-01)
In this study we utilized the concept of rational drug design to identify novel compounds with optimal selectivity, efficacy and safety, which would bind to the target enzyme pteridine reductase 1 (PTR1) in Leishmania parasites. Twelve compounds afforded from Baylis-Hillman



Global Trade Item Number

SKUGTIN
S7899-500ML04061836960421
S7899-100ML04061836960407
S7899-1L04061836960414

Questions

1–3 of 3 Questions  
  1. what is the proper way to store the Sodium acetate buffer solution and how long can I keep it without losing its properties after opening;

    1 answer
    1. The product is chemically stable under standard ambient conditions (room temperature). Section 7 of the Safety Data Sheet provides information on handling and storage. The product is covered under a 1-year warranty if stored correctly. https://www.sigmaaldrich.com/sds/sigma/s7899?userType=anonymous

      Helpful?

  2. What is the concentration of the acetic acid in this solution?

    1 answer
    1. This product is prepared from a powdered sodium acetate compound. The material is CAS 127-09-3 with the molecular formula of C2H3NaO2. No additional acetic acid is used in the formulation of this buffer.

      Helpful?

  3. 여기 용액안에 Triton-X100가 들어가 있나요?

    1 answer
    1. As mentioned on the Product Datasheet located in the 'DOCUMENTATION' section, this product is a 3 M solution prepared with sodium acetate (Molecular Biology grade) and 18 megaohm water. Triton-X 100 has not been added to this formulation.

      Helpful?

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