S2657

Anti-S-100 (β-Subunit) antibody, Mouse monoclonal

clone SH-B4, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse, Anti-NEF, Anti-S100-B
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00162834
• Conjugate: unconjugated
• Clone: SH-B4, monoclonal
• Application: ELISA (i), IHC (p), WB
• Citations: 18

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: (Solution in 0.01M phosphate buffered saline pH 7.4, containing 15mM sodium azide as a preservative)
• antibody product type: primary antibodies
• clone: SH-B4, monoclonal
• contains: 15 mM sodium azide
• species reactivity: sheep, bovine, goat, pig, feline, rabbit, rat, canine, human
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable (: suitable), indirect ELISA: suitable (:suitable), western blot: 1:100-1:200 ( (using S-100B Protein, Bovine Brain))
• isotype: IgG1
• UniProt accession no.: P04271
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... S100B(6285) ; rat ... S100b(25742)

Description

General description

Monoclonal Anti-S-100 (b-Subunit) (mouse IgG1 isotype) is derived from the SH-B4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. S-100 is a set of small, highly acidic, thermolabile dimer proteins of ~20 kDa, widely distributed in different tissues.

S-100 (β-Subunit) is a calcium-binding protein, that belongs to S100 family of calcium-binding proteins. It is expressed in melanocytes. It is located on human chromosome 21q22.3.

Immunogen

bovine brain S-100b

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)

Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse has been used in immunocytochemistry and immunohistochemistry. It has also been used as a component in serum medium for 5-ethynyl-29-deoxyuridine (EdU), incorporation assay.

Biochem/physiol Actions

Recognizes an epitope located on the β chain (i.e. in S-100a and S-100b) but not on the α chain of S-100 (i.e. in S-100a and S-100ao). In ELISA, recognition of S-100 subunit by clone SH-B4 is Ca²⁺-dependent. The product does not react with other members of the EF-hand family such as, calmodulin, parvalbumin, intestinal calcium-binding protein and myosin light chain. In immunohistochemistry, the antibody detects normal and neoplastic S-100 β subunit-containing cells (e.g. Schwann cells, chondrocytes, melanocytes, and melanotic tumors) in protease-digested, formalin-fixed, paraffin-embedded tissues.

S-100 (β-Subunit) acts as a marker of melanocyte cytotoxicity. Reduced expression of S100B is observed in chronic liver diseases. It also acts as a serum marker in endocrine resistant breast cancer.

S-100 is considered to be a cell-growth regulator, but other functions have been suggested, e.g., increasing the membrane permeability to cations under physiologic conditions, stimulation of nucleolar RNA polymerase activity, interaction with the tumor suppressor protein p53 and as a carrier of proteins and free fatty acids in adipocytes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• wgk: WGK 1