PEROX1

Peroxisome Isolation Kit

isolate peroxisomes from tissues and cells

• Synonym(s): Isolation Kit for Peroxisomes, Kit for Peroxisomes, Peroxisome Kit
• NACRES: NA.32
• UNSPSC Code: 12352200

Properties

• Quality Segment: 200
• technique(s): centrifugation: suitable, fractionation: suitable
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Isolated peroxisomes are used for studying lipid β-oxidation,[1] amino acid metabolism[2] and biosynthesis of ether-linked glycerolipids[3] and bile acids.[4][5]

Application

The Perixosome Isolation Kit provides all the necessary reagents and a detailed protocol for the isolation of highly purified peroxisomes from animal tissues and cells, by differential density gradient centrifugation using iodixanol [OptiPrep^™]. This kit has been used for preparation of peroxisomes from rat liver, rat kidney and rabbit liver as well as HEK293 and HepG2 cells.

Features and Benefits

• Specially formulated extraction reagents for research scale applications - save time and minimize waste
• Produces functional intact organelles - resulting peroxisomes are suitable for functional studies, metabolic assays, protein profiling, and disease state analysis
• Compatible with products for structure confirmation - easily confirm intactness with companion test kit, Cytochrome C Reductase Assay Kit (Cat. No. CY0100)

Other Notes

Upon receiving the kit, the Protease Inhibitor Cocktail (Product Code P 8340) should be stored at –20 °C and the OptiPrep Density Gradient Medium (Product Code O3028) should be stored at room temperature.

Legal Information

OptiPrep is a trademark of Serumwerk Bernburg AG

Kit Components Also Available Separately

• P8340

  Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution

  SDS

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290,H315,H319
• pcodes: P234 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Hazard Classifications: Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
• flash_point_f: 188.6 °F - closed cup
• flash_point_c: 87 °C - closed cup
• wgk: WGK 2
• Storage Class: 10 - Combustible liquids