P8913
Phospholipase A2 from bovine pancreas
lyophilized powder, ≥20 units/mg protein
Synonym(s):
Lecithinase A, PLA2, Phosphatidylcholine 2-acylhydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
232-637-7
MDL number:
Specific activity:
≥20 units/mg protein
Pricing and availability is not currently available.
form
lyophilized powder
Quality Segment
specific activity
≥20 units/mg protein
composition
Protein, 5-20% biuret
UniProt accession no.
storage temp.
−20°C
Gene Information
cow ... PLA2G1B(282457)
General description
Phospholipase A2 from bovine pancreas is a calcium-dependent lipolytic enzyme.[1]
Application
Phospholipase A2 from bovine pancreas has been used to determine rat renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation and to study the impact of phospholipase A2 on tubular cell triglyceride levels.
Biochem/physiol Actions
Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.
Packaging
Package size based on protein content
Physical form
Contains Tris buffer salts.
Analysis Note
Protein determined by biuret.
Other Notes
One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.0 and 37 °C.
1 of 1
This Item | |||
|---|---|---|---|
| Gene Information cow ... PLA2G1B(282457) | Gene Information pig ... PLA2G1B(445525) | Gene Information - | Gene Information cow ... TRYP8(282603) |
| specific activity ≥20 units/mg protein | specific activity ≥600 units/mg protein | specific activity ≥200 units/g protein | specific activity ≥8,500 BAEE units/mg protein (biuret) |
| form lyophilized powder | form ammonium sulfate suspension | form lyophilized powder | form powder |
| UniProt accession no. | UniProt accession no. | UniProt accession no. - | UniProt accession no. |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
wgk
WGK 1
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Product Information Sheet
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P8913-5MG | 04061834402084 |
| P8913-10MG | 04061834402060 |
| P8913-50MG | 04061833273890 |