P7288

o-Phenylenediamine dihydrochloride

chromogenic, tablet

• Synonym(s): 1,2-Phenylenediamine tablet, OPD, OPD Tablet
• Linear Formula: C₆H₄(NH₂)₂·2HCl
• CAS Number: 615-28-1
• Molecular Weight: 181.06
• NACRES: NA.83
• PubChem Substance ID: 24898837
• UNSPSC Code: 12352204
• EC Number: 210-418-7
• MDL number: MFCD00012966
• Beilstein/REAXYS Number: 3912045

Properties

• Product Name: o-Phenylenediamine dihydrochloride, tablet, 20 mg substrate per tablet
• Quality Segment: 200
• form: tablet
• mp: 258 °C (dec.) (lit.)
• storage temp.: 2-8°C
• SMILES string: Cl[H].Cl[H].Nc1ccccc1N
• InChI: 1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
• InChI key: RIIWUGSYXOBDMC-UHFFFAOYSA-N

Description

General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Application

o-Phenylenediamine dihydrochloride has been used:

• as a substrate for the eosinophil peroxidase (EPO) assay in bronchoalveolar lavage fluid (BALF)
• in cell adhesion molecule-enzyme immunoassay (CAM-EIA) to analyze the amounts of E-selectin induced by test compounds (LPS or solutions and materials) on endothelial cells
• in ultra-sensitive enzyme-linked immunosorbent assay (ELISA) to measure prolactin and luteinizing hormone (LH)

Preparation Note

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Safety Information

• pictograms: GHS08,GHS07,GHS09
• signalword: Warning
• hcodes: H302 + H332,H317,H319,H341,H351,H410
• pcodes: P273 - P280 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• wgk: WGK 3