N0142

Monoclonal Anti-Neurofilament 200 (Phos. and Non-Phos.) antibody produced in mouse

clone N52, ascites fluid

• Synonym(s): Anti-CMT2CC, Anti-NFH
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00164708
• Conjugate: unconjugated
• Clone: N52, monoclonal
• Application: ARR, IHC (f), IHC (p), WB
• Citations: 311

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: N52, monoclonal
• contains: 15 mM sodium azide
• species reactivity: wide range
• packaging: antibody small pack of 25 μL
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunohistochemistry (frozen sections): 1:400 using rat cerebellum, microarray: suitable, western blot: 1:1,000 using rat brain extract
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NEFH(4744) ; mouse ... Nefh(380684) ; rat ... Nefh(24587)

Description

General description

Neurofilaments are composed of three subunits, namely NEFL (light), NEFM (medium) and NEFH (heavy). NEFH (neurofilament heavy) gene encodes the most important neuron-specific intermediate filament of cytoskeleton in myelinated axons. This gene is mapped to human chromosome 22q12.2.
Monoclonal Anti-Neurofilament 200 (phosphorylated and non-phosphorylated) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

C-terminal segment of enzymatically dephosphorylated pig neurofilament 200.

Application

Monoclonal Anti-Neurofilament 200 (Phos. and Non-Phos.) antibody has been used in immunohistochemistry and western blotting.

Biochem/physiol Actions

Displays a broad species cross-reactivity, recognizing both the phosphorylated and non-phosphorylated forms of this neural specific antigen using various techniques.

The heavy polypeptide neurofilament encoded by neurofilament heavy chain (NEFH) gene participates in the maintenance of cytoskeleton and axonal architecture in the proximal axonal region of spinal motoneurons. Mutations in the NEFH gene has a key role in the pathogenesis of sporadic amyotrophic lateral sclerosis (ALS). NEFH gene is also identified to generate autosomal dominant axonal Charcot-Marie-tooth disease (CMT2cc).

Physical form

Supplied as ascites fluid containing 15 mM sodium azide as a preservative.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable